Sensitive assay of Escherichia coli in food samples by microchip capillary electrophoresis based on specific aptamer binding strategy.
Aptamer
Drinking water and milk samples
Escherichia coli
Microchip capillary electrophoresis
Journal
Talanta
ISSN: 1873-3573
Titre abrégé: Talanta
Pays: Netherlands
ID NLM: 2984816R
Informations de publication
Date de publication:
15 May 2019
15 May 2019
Historique:
received:
17
08
2018
revised:
07
01
2019
accepted:
09
01
2019
entrez:
18
2
2019
pubmed:
18
2
2019
medline:
9
4
2019
Statut:
ppublish
Résumé
The rapid and cost-effective detection of bacteria is of great importance to ensuring food safety, preventing food poisoning. Herein, we developed a sensitive detection of Escherichia coli (E. coli) using bacteria-specific aptamer in conjunction with microchip capillary electrophoresis-coupled laser-induced fluorescence (MCE-LIF). Based on the differences between charge to mass ratios of free aptamer and bacteria-aptamer complex, which influence their electrophoretic mobilities, the separation of free aptamers and complex peaks by MCE could be achieved. Under optimal conditions, the sensitive detection of E. coli was achieved with a detection limit of 3.7 × 10
Identifiants
pubmed: 30771937
pii: S0039-9140(19)30041-4
doi: 10.1016/j.talanta.2019.01.040
pii:
doi:
Substances chimiques
Aptamers, Nucleotide
0
Drinking Water
0
Fluorescent Dyes
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
284-290Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.