Technical note: Bovine adipocyte and preadipocyte co-culture as an efficient adipogenic model.
adipocytes
adipogenesis
bovine
co-culture
in vitro
Journal
Journal of dairy science
ISSN: 1525-3198
Titre abrégé: J Dairy Sci
Pays: United States
ID NLM: 2985126R
Informations de publication
Date de publication:
Apr 2019
Apr 2019
Historique:
received:
30
08
2018
accepted:
14
12
2018
pubmed:
18
2
2019
medline:
1
5
2019
entrez:
18
2
2019
Statut:
ppublish
Résumé
Reductionist studies of adipose tissue biology require reliable in vitro adipocyte culturing models. Current protocols for adipogenesis induction in stromal vascular fraction-derived preadipocytes require extended culturing periods and have low adipogenic rates. We compared the adipogenic efficiency of a 7-d co-culture model of visceral (VIS) and subcutaneous (SC) stromal vascular fraction-derived preadipocytes with mature adipocytes with a 14-d standard adipocyte differentiation protocol. We obtained preadipocytes and mature adipocytes from SC and VIS adipose tissue of nonlactating, nongestating Holstein cows (n = 6). Adipogenesis induction was performed using a standard protocol for 7 (SD7; control) or 14 d (SD14), and a co-culture model for 7 d (CC7). Culture conditions, including medium composition, were the same for all treatments. For CC7, 900 primary adipocytes/cm
Identifiants
pubmed: 30772027
pii: S0022-0302(19)30153-5
doi: 10.3168/jds.2018-15626
pii:
doi:
Substances chimiques
Isoproterenol
L628TT009W
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3622-3629Informations de copyright
Copyright © 2019 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.