Peripheral expression of long non-coding RNAs in bipolar patients.


Journal

Journal of affective disorders
ISSN: 1573-2517
Titre abrégé: J Affect Disord
Pays: Netherlands
ID NLM: 7906073

Informations de publication

Date de publication:
15 Apr 2019
Historique:
received: 12 11 2018
revised: 13 01 2019
accepted: 11 02 2019
pubmed: 18 2 2019
medline: 14 6 2019
entrez: 18 2 2019
Statut: ppublish

Résumé

Long non-coding RNAs (lncRNAs) have established roles in the pathogenesis of diverse human disorders including neuropsychiatric disorders. In the current study, we evaluated expression levels of six apoptosis-related lncRNAs (CCAT2, TUG1, PANDA, NEAT1, FAS-AS1 and OIP5-AS1) in the peripheral blood of bipolar disorder (BD) patients and healthy subjects to assess their contribution in the pathogenesis of BD. CCAT2, TUG1 and PANDA were up-regulated in total BD patients compared with total healthy subjects (P values = 0.006, <0.001 and 0.004 respectively) while OIP5-AS1 was down-regulated (P = 0.001). When expression levels of these genes were compared between patients and sex-matched healthy subjects, CCAT2 and TUG1 expression levels were only different in male subgroups; while PANDA expression was different in both male and female subgroups compared with the corresponding control subgroups. Transcript levels of lncRNAs were not correlated with any of demographic or clinical parameters of BD patients or controls after adjustment for gender. Pairwise correlations between expression levels of lncRNAs followed a disease-dependent manner. Based on receiver operating characteristic curves, among the assessed lncRNAs TUG1 had the highest diagnostic power in BD. Combination of transcript levels of CCAT2, TUG1, PANDA and OIP5-AS1 improved both sensitivity and specificity resulting in diagnostic power of 0.96. Our data demonstrated a possible role of certain lncRNAs in the pathogenesis of BD and potentiated them as diagnostic markers in this disorder.

Sections du résumé

BACKGROUND BACKGROUND
Long non-coding RNAs (lncRNAs) have established roles in the pathogenesis of diverse human disorders including neuropsychiatric disorders.
METHODS METHODS
In the current study, we evaluated expression levels of six apoptosis-related lncRNAs (CCAT2, TUG1, PANDA, NEAT1, FAS-AS1 and OIP5-AS1) in the peripheral blood of bipolar disorder (BD) patients and healthy subjects to assess their contribution in the pathogenesis of BD.
RESULTS RESULTS
CCAT2, TUG1 and PANDA were up-regulated in total BD patients compared with total healthy subjects (P values = 0.006, <0.001 and 0.004 respectively) while OIP5-AS1 was down-regulated (P = 0.001). When expression levels of these genes were compared between patients and sex-matched healthy subjects, CCAT2 and TUG1 expression levels were only different in male subgroups; while PANDA expression was different in both male and female subgroups compared with the corresponding control subgroups. Transcript levels of lncRNAs were not correlated with any of demographic or clinical parameters of BD patients or controls after adjustment for gender. Pairwise correlations between expression levels of lncRNAs followed a disease-dependent manner. Based on receiver operating characteristic curves, among the assessed lncRNAs TUG1 had the highest diagnostic power in BD. Combination of transcript levels of CCAT2, TUG1, PANDA and OIP5-AS1 improved both sensitivity and specificity resulting in diagnostic power of 0.96.
CONCLUSION CONCLUSIONS
Our data demonstrated a possible role of certain lncRNAs in the pathogenesis of BD and potentiated them as diagnostic markers in this disorder.

Identifiants

pubmed: 30772744
pii: S0165-0327(18)32884-2
doi: 10.1016/j.jad.2019.02.034
pii:
doi:

Substances chimiques

Biomarkers 0
RNA, Long Noncoding 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

169-174

Informations de copyright

Copyright © 2019 Elsevier B.V. All rights reserved.

Auteurs

Arezou Sayad (A)

Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Mohammad Taheri (M)

Student Research Committee, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address: Mohammad.taheri@sbmu.ac.ir.

Mir Davood Omrani (MD)

Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Hamid Fallah (H)

Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Vahid Kholghi Oskooei (V)

Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Soudeh Ghafouri-Fard (S)

Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address: s.ghafourifard@sbmu.ac.ir.

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Classifications MeSH