Microinjection of oocytes and embryos with synthetic mRNA encoding molecular probes.
Echinoderm
Embryo
Fluorescent protein
Microinjection
Oocyte
Synthetic mRNA
Journal
Methods in cell biology
ISSN: 0091-679X
Titre abrégé: Methods Cell Biol
Pays: United States
ID NLM: 0373334
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
20
2
2019
pubmed:
20
2
2019
medline:
20
6
2019
Statut:
ppublish
Résumé
We describe methods and techniques for introduction of molecular probes in the form of synthetic mRNA by rapid repetitive microinjection into oocytes or early embryos of echinoderms and various invertebrates. Construct assembly is followed by standard kit-based in vitro mRNA synthesis, with slight modifications to optimize expression and clean-up. Variations of a basic microinjection procedures are detailed for echinoderms: starfish oocytes (Patiria miniata or other species), purple urchin (Strongylocentrotus purpuratus) and sand dollar (Dendraster excentricus) zygotes, with notes included for other invertebrate eggs and embryos as well.
Identifiants
pubmed: 30777176
pii: S0091-679X(18)30150-X
doi: 10.1016/bs.mcb.2018.10.012
pii:
doi:
Substances chimiques
Molecular Probes
0
RNA, Messenger
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
189-222Informations de copyright
© 2019 Elsevier Inc. All rights reserved.