Indications for a moonlighting function of translation factor aIF5A in the crenarchaeum Sulfolobus solfataricus.

Archaeal Proteins / genetics CRISPR-Cas Systems Peptide Initiation Factors / genetics Protein Biosynthesis RNA, Archaeal / metabolism RNA-Binding Proteins / genetics Sulfolobus solfataricus / growth & development Eukaryotic Translation Initiation Factor 5A
None Archaea RNase Translation factor aIF5A

Journal

RNA biology
ISSN: 1555-8584
Titre abrégé: RNA Biol
Pays: United States
ID NLM: 101235328

Informations de publication

Date de publication:
05 2019
Historique:
pmc-release: 05 03 2020
pubmed: 20 2 2019
medline: 27 11 2019
entrez: 20 2 2019
Statut: ppublish

Résumé

Translation factor a/eIF5A is highly conserved in Eukarya and Archaea. The eukaryal eIF5A protein is required for transit of ribosomes across consecutive proline codons, whereas the function of the archaeal orthologue remains unknown. Here, we provide a first hint for an involvement of Sulfolobus solfataricus (Sso) aIF5A in translation. CRISPR-mediated knock down of the aif5A gene resulted in strong growth retardation, underlining a pivotal function. Moreover, in vitro studies revealed that Sso aIF5A is endowed with endoribonucleolytic activity. Thus, aIF5A appears to be a moonlighting protein that might be involved in protein synthesis as well as in RNA metabolism.

Identifiants

DOI: 10.1080/15476286.2019.1582953 PMID: 30777488 PMC: PMC6546411
pubmed: 30777488
doi: 10.1080/15476286.2019.1582953
pmc: PMC6546411
doi:

Substances chimiques

Archaeal Proteins 0
Peptide Initiation Factors 0
RNA, Archaeal 0
RNA-Binding Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

675-685

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Auteurs

Flavia Bassani (F)

a Department of Microbiology, Immunobiology and Genetics, Max F. Perutz Laboratories , University of Vienna , Vienna , Austria.

Isabelle Anna Zink (IA)

b Division of Archaea Biology and Ecogenomics, Department of Ecogenomics and Systems Biology , University of Vienna , Vienna , Austria.

Thomas Pribasnig (T)

b Division of Archaea Biology and Ecogenomics, Department of Ecogenomics and Systems Biology , University of Vienna , Vienna , Austria.

Michael T Wolfinger (MT)

c Institute of Theoretical Chemistry , University of Vienna , Vienna , Austria.
ORCID: 0000-0003-0925-5205

Alice Romagnoli (A)

d Department of Life and Environmental Sciences , Polytechnic University of Marche , Ancona , Italy.

Armin Resch (A)

a Department of Microbiology, Immunobiology and Genetics, Max F. Perutz Laboratories , University of Vienna , Vienna , Austria.

Christa Schleper (C)

b Division of Archaea Biology and Ecogenomics, Department of Ecogenomics and Systems Biology , University of Vienna , Vienna , Austria.

Udo Bläsi (U)

a Department of Microbiology, Immunobiology and Genetics, Max F. Perutz Laboratories , University of Vienna , Vienna , Austria.

Anna La Teana (A)

d Department of Life and Environmental Sciences , Polytechnic University of Marche , Ancona , Italy.
ORCID: 0000-0001-6232-0497

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines d'archée › Indications for a moonlighting function of...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Systèmes CRISPR-Cas › Indications for a moonlighting function of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines ribosomiques › Facteurs initiation chaîne peptidique › Indications for a moonlighting function of...
questionsmedicales.fr › Phénomènes génétiques › Expression des gènes › Biosynthèse des protéines › Indications for a moonlighting function of...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN des archées › Indications for a moonlighting function of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Nucléoprotéines › Protéines de liaison à l'ARN › Indications for a moonlighting function of...
questionsmedicales.fr › Archéobactéries › Crenarchaeota › Sulfolobale › Sulfolobaceae › Sulfolobus › Sulfolobus solfataricus › Indications for a moonlighting function of...