Lung regeneration by multipotent stem cells residing at the bronchioalveolar-duct junction.
Animals
Bronchioles
/ physiology
Bronchoalveolar Lavage Fluid
/ cytology
Cell Differentiation
/ genetics
Cell Lineage
/ genetics
Cells, Cultured
Epithelial Cells
/ physiology
Genotype
Lung
/ physiology
Mice
Mice, Inbred C57BL
Mice, Transgenic
Multipotent Stem Cells
/ physiology
Regeneration
/ genetics
Journal
Nature genetics
ISSN: 1546-1718
Titre abrégé: Nat Genet
Pays: United States
ID NLM: 9216904
Informations de publication
Date de publication:
04 2019
04 2019
Historique:
received:
20
02
2018
accepted:
09
01
2019
pubmed:
20
2
2019
medline:
20
4
2019
entrez:
20
2
2019
Statut:
ppublish
Résumé
Characterizing the stem cells responsible for lung repair and regeneration is important for the treatment of pulmonary diseases. Recently, a unique cell population located at the bronchioalveolar-duct junctions has been proposed to comprise endogenous stem cells for lung regeneration. However, the role of bronchioalveolar stem cells (BASCs) in vivo remains debated, and the contribution of such cells to lung regeneration is not known. Here we generated a genetic lineage-tracing system that uses dual recombinases (Cre and Dre) to specifically track BASCs in vivo. Fate-mapping and clonal analysis showed that BASCs became activated and responded distinctly to different lung injuries, and differentiated into multiple cell lineages including club cells, ciliated cells, and alveolar type 1 and type 2 cells for lung regeneration. This study provides in vivo genetic evidence that BASCs are bona fide lung epithelial stem cells with deployment of multipotency and self-renewal during lung repair and regeneration.
Identifiants
pubmed: 30778223
doi: 10.1038/s41588-019-0346-6
pii: 10.1038/s41588-019-0346-6
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
728-738Commentaires et corrections
Type : ErratumIn
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