LHCSR3 is a nonphotochemical quencher of both photosystems in
Algal Proteins
/ genetics
Chlamydomonas reinhardtii
/ genetics
Chlorophyll
/ metabolism
Light-Harvesting Protein Complexes
/ genetics
Luminescent Proteins
/ genetics
Photosynthesis
/ physiology
Photosystem I Protein Complex
/ metabolism
Photosystem II Protein Complex
/ genetics
Protein Kinases
/ metabolism
Temperature
Zeaxanthins
/ metabolism
LHCSR
NPQ
microalgae
photoprotection
photosynthesis
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
05 03 2019
05 03 2019
Historique:
pubmed:
21
2
2019
medline:
24
3
2020
entrez:
21
2
2019
Statut:
ppublish
Résumé
Photosynthetic organisms prevent oxidative stress from light energy absorbed in excess through several photoprotective mechanisms. A major component is thermal dissipation of chlorophyll singlet excited states and is called nonphotochemical quenching (NPQ). NPQ is catalyzed in green algae by protein subunits called LHCSRs (Light Harvesting Complex Stress Related), homologous to the Light Harvesting Complexes (LHC), constituting the antenna system of both photosystem I (PSI) and PSII. We investigated the role of LHCSR1 and LHCSR3 in NPQ activation to verify whether these proteins are involved in thermal dissipation of PSI excitation energy, in addition to their well-known effect on PSII. To this aim, we measured the fluorescence emitted at 77 K by whole cells in a quenched or unquenched state, using green fluorescence protein as the internal standard. We show that NPQ activation by high light treatment in
Identifiants
pubmed: 30782831
pii: 1809812116
doi: 10.1073/pnas.1809812116
pmc: PMC6410775
doi:
Substances chimiques
Algal Proteins
0
Light-Harvesting Protein Complexes
0
Luminescent Proteins
0
Photosystem I Protein Complex
0
Photosystem II Protein Complex
0
Zeaxanthins
0
Chlorophyll
1406-65-1
Protein Kinases
EC 2.7.-
light-harvesting complex II kinase
EC 2.7.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4212-4217Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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