Decoupling the Roles of Cell Shape and Mechanical Stress in Orienting and Cueing Epithelial Mitosis.
Xenopus
cell division
cell proliferation
cell shape
epithelium
force
mechanical stress
mitosis
mitotic spindle
vertex model
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
19 02 2019
19 02 2019
Historique:
received:
23
09
2017
revised:
11
12
2018
accepted:
28
01
2019
entrez:
21
2
2019
pubmed:
21
2
2019
medline:
11
4
2020
Statut:
ppublish
Résumé
Distinct mechanisms involving cell shape and mechanical force are known to influence the rate and orientation of division in cultured cells. However, uncoupling the impact of shape and force in tissues remains challenging. Combining stretching of Xenopus tissue with mathematical methods of inferring relative mechanical stress, we find separate roles for cell shape and mechanical stress in orienting and cueing division. We demonstrate that division orientation is best predicted by an axis of cell shape defined by the position of tricellular junctions (TCJs), which align with local cell stress rather than tissue-level stress. The alignment of division to cell shape requires functional cadherin and the localization of the spindle orientation protein, LGN, to TCJs but is not sensitive to relative cell stress magnitude. In contrast, proliferation rate is more directly regulated by mechanical stress, being correlated with relative isotropic stress and decoupled from cell shape when myosin II is depleted.
Identifiants
pubmed: 30784591
pii: S2211-1247(19)30139-1
doi: 10.1016/j.celrep.2019.01.102
pmc: PMC6381790
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2088-2100.e4Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 105610/Z/14/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 098390/Z/12/Z
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
Pays : United Kingdom
Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.
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