A DNA repair protein and histone methyltransferase interact to promote genome stability in the Caenorhabditis elegans germ line.
Journal
PLoS genetics
ISSN: 1553-7404
Titre abrégé: PLoS Genet
Pays: United States
ID NLM: 101239074
Informations de publication
Date de publication:
02 2019
02 2019
Historique:
received:
22
06
2018
accepted:
28
01
2019
revised:
06
03
2019
pubmed:
23
2
2019
medline:
29
3
2019
entrez:
23
2
2019
Statut:
epublish
Résumé
Histone modifications regulate gene expression and chromosomal events, yet how histone-modifying enzymes are targeted is poorly understood. Here we report that a conserved DNA repair protein, SMRC-1, associates with MET-2, the C. elegans histone methyltransferase responsible for H3K9me1 and me2 deposition. We used molecular, genetic, and biochemical methods to investigate the biological role of SMRC-1 and to explore its relationship with MET-2. SMRC-1, like its mammalian ortholog SMARCAL1, provides protection from DNA replication stress. SMRC-1 limits accumulation of DNA damage and promotes germline and embryonic viability. MET-2 and SMRC-1 localize to mitotic and meiotic germline nuclei, and SMRC-1 promotes an increase in MET-2 abundance in mitotic germline nuclei upon replication stress. In the absence of SMRC-1, germline H3K9me2 generally decreases after multiple generations at high culture temperature. Genetic data are consistent with MET-2 and SMRC-1 functioning together to limit replication stress in the germ line and in parallel to promote other germline processes. We hypothesize that loss of SMRC-1 activity causes chronic replication stress, in part because of insufficient recruitment of MET-2 to nuclei.
Identifiants
pubmed: 30794539
doi: 10.1371/journal.pgen.1007992
pii: PGENETICS-D-18-01292
pmc: PMC6402707
doi:
Substances chimiques
Caenorhabditis elegans Proteins
0
Histones
0
Histone-Lysine N-Methyltransferase
EC 2.1.1.43
Met-2 protein, C elegans
EC 2.1.1.43
DNA Helicases
EC 3.6.4.-
smrc-1 protein, C elegans
EC 3.6.4.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1007992Subventions
Organisme : NIH HHS
ID : P40 OD010440
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM089818
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM104007
Pays : United States
Organisme : NIGMS NIH HHS
ID : R15 GM119029
Pays : United States
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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