Rationally designed azobenzene photoswitches for efficient two-photon neuronal excitation.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
22 02 2019
22 02 2019
Historique:
received:
03
05
2018
accepted:
24
01
2019
entrez:
24
2
2019
pubmed:
24
2
2019
medline:
4
4
2019
Statut:
epublish
Résumé
Manipulation of neuronal activity using two-photon excitation of azobenzene photoswitches with near-infrared light has been recently demonstrated, but their practical use in neuronal tissue to photostimulate individual neurons with three-dimensional precision has been hampered by firstly, the low efficacy and reliability of NIR-induced azobenzene photoisomerization compared to one-photon excitation, and secondly, the short cis state lifetime of the two-photon responsive azo switches. Here we report the rational design based on theoretical calculations and the synthesis of azobenzene photoswitches endowed with both high two-photon absorption cross section and slow thermal back-isomerization. These compounds provide optimized and sustained two-photon neuronal stimulation both in light-scattering brain tissue and in Caenorhabditis elegans nematodes, displaying photoresponse intensities that are comparable to those achieved under one-photon excitation. This finding opens the way to use both genetically targeted and pharmacologically selective azobenzene photoswitches to dissect intact neuronal circuits in three dimensions.
Identifiants
pubmed: 30796228
doi: 10.1038/s41467-019-08796-9
pii: 10.1038/s41467-019-08796-9
pmc: PMC6385291
doi:
Substances chimiques
Azo Compounds
0
Calcium Channels
0
azobenzene
F0U1H6UG5C
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
907Références
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