4-O-Methylascochlorin inhibits the prolyl hydroxylation of hypoxia-inducible factor-1α, which is attenuated by ascorbate.
Journal
The Journal of antibiotics
ISSN: 1881-1469
Titre abrégé: J Antibiot (Tokyo)
Pays: England
ID NLM: 0151115
Informations de publication
Date de publication:
05 2019
05 2019
Historique:
received:
06
08
2018
accepted:
31
01
2019
revised:
14
01
2019
pubmed:
24
2
2019
medline:
30
4
2019
entrez:
24
2
2019
Statut:
ppublish
Résumé
4-O-Methylascochlorin (MAC), a methylated derivative of ascochlorin, was previously shown to promote the accumulation of hypoxia-inducible factor (HIF)-1α in human breast adenocarcinoma MCF-7 cells. In the present study, we further investigated the effects of MAC on the expression and function of HIF-1α in human fibrosarcoma HT-1080 cells. MAC promoted the accumulation of the HIF-1α protein without affecting its constitutive mRNA expression and augmented the transcriptional activation of HIF target genes. Ascorbate, but not N-acetylcysteine, attenuated MAC-mediated HIF-1α accumulation. MAC-induced increases in HIF-1α transcriptional activity were also attenuated by ascorbate. MAC inhibited the hydroxylation of HIF-1α at the proline 564 residue, while it was reversed by ascorbate. MAC slightly decreased the intracellular concentration of ascorbate. The present results demonstrated that MAC promoted the accumulation of HIF-1α by preventing prolyl hydroxylation, and ascorbate attenuated the MAC-mediated inhibition of HIF-1α prolyl hydroxylation.
Identifiants
pubmed: 30796332
doi: 10.1038/s41429-019-0157-0
pii: 10.1038/s41429-019-0157-0
doi:
Substances chimiques
Enzyme Inhibitors
0
HIF1A protein, human
0
Hypoxia-Inducible Factor 1, alpha Subunit
0
Terpenes
0
4-O-methylascochlorin
38561-40-9
Proline
9DLQ4CIU6V
Ascorbic Acid
PQ6CK8PD0R
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
271-281Subventions
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : 25292061
Pays : International
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : 16H04910
Pays : International
Commentaires et corrections
Type : ErratumIn
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