Light-sheet fluorescence expansion microscopy: fast mapping of neural circuits at super resolution.
connectomics
dentate gyrus
light-sheet fluorescence microscopy
super resolution
tissue expansion
Journal
Neurophotonics
ISSN: 2329-423X
Titre abrégé: Neurophotonics
Pays: United States
ID NLM: 101632875
Informations de publication
Date de publication:
Jan 2019
Jan 2019
Historique:
received:
08
08
2018
accepted:
09
01
2019
entrez:
24
2
2019
pubmed:
24
2
2019
medline:
24
2
2019
Statut:
ppublish
Résumé
The goal of understanding the architecture of neural circuits at the synapse level with a brain-wide perspective has powered the interest in high-speed and large field-of-view volumetric imaging at subcellular resolution. Here, we developed a method combining tissue expansion and light-sheet fluorescence microscopy to allow extended volumetric super resolution high-speed imaging of large mouse brain samples. We demonstrate the capabilities of this method by performing two color fast volumetric super resolution imaging of mouse CA1 and dentate gyrus molecular-, granule cell-, and polymorphic layers. Our method enables an exact evaluation of granule cell and neurite morphology within the context of large cell ensembles spanning several orders of magnitude in resolution. We found that imaging a brain region of
Identifiants
pubmed: 30796881
doi: 10.1117/1.NPh.6.1.015005
pii: 18043RR
pmc: PMC6368534
doi:
Types de publication
Journal Article
Langues
eng
Pagination
015005Références
Science. 2004 Aug 13;305(5686):1007-9
pubmed: 15310904
Nat Methods. 2005 Dec;2(12):920-31
pubmed: 16299477
Nat Methods. 2005 Dec;2(12):932-40
pubmed: 16299478
Nat Methods. 2007 Apr;4(4):331-6
pubmed: 17384643
J Neurosci. 2007 Sep 12;27(37):9817-23
pubmed: 17855595
Bioinformatics. 2009 Jun 1;25(11):1463-5
pubmed: 19346324
Annu Rev Cell Dev Biol. 2010;26:285-314
pubmed: 20929313
Neuron. 2010 Nov 18;68(4):639-53
pubmed: 21092855
Curr Opin Neurobiol. 2012 Feb;22(1):138-43
pubmed: 21925871
Nat Methods. 2012 Jan 15;9(3):255-8
pubmed: 22245809
Proc Natl Acad Sci U S A. 2012 May 29;109(22):8434-9
pubmed: 22586078
Opt Express. 2012 Aug 27;20(18):20582-98
pubmed: 23037106
Opt Express. 2012 Sep 10;20(19):21805-14
pubmed: 23037300
Nature. 2013 May 16;497(7449):332-7
pubmed: 23575631
Nat Methods. 2013 Jun;10(6):515-23
pubmed: 23722211
Trends Cogn Sci. 2013 Dec;17(12):596-9
pubmed: 24210964
Laser Photon Rev. 2013 Sep;7(5):732-757
pubmed: 24348874
Science. 2014 Oct 24;346(6208):1257998
pubmed: 25342811
Nat Methods. 2015 Jan;12(1):23-6
pubmed: 25549266
Science. 2015 Jan 30;347(6221):543-8
pubmed: 25592419
Science. 2015 Jan 30;347(6221):474-5
pubmed: 25635071
Nat Methods. 2015 Jun;12(6):541-6
pubmed: 25867849
PLoS One. 2015 May 20;10(5):e0124650
pubmed: 25993380
Cell. 2015 Jul 16;162(2):246-257
pubmed: 26186186
Nat Methods. 2016 Jun;13(6):485-8
pubmed: 27064647
Annu Rev Cell Dev Biol. 2016 Oct 6;32:713-741
pubmed: 27298088
Nat Biotechnol. 2016 Sep;34(9):987-92
pubmed: 27376584
Neurosci Bull. 2017 Feb;33(1):95-102
pubmed: 27535148
Nat Commun. 2017 Jan 19;8:14162
pubmed: 28102196
Nat Methods. 2017 Mar 31;14(4):360-373
pubmed: 28362435
Nat Methods. 2017 Jun;14(6):593-599
pubmed: 28417997
Mol Brain. 2017 Jul 20;10(1):33
pubmed: 28728585
Eur J Neurosci. 2018 May;47(9):1033-1042
pubmed: 29512842
J Microsc. 1993 Jun;170(Pt 3):229-36
pubmed: 8371260