Isolation of Tumor-Infiltrating Lymphocytes by Ficoll-Paque Density Gradient Centrifugation.
Immune escape
Immunotherapy
Mechanical dissociation
Tumor microenvironment
Tumor-infiltrating lymphocytes
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
25
2
2019
pubmed:
25
2
2019
medline:
28
7
2019
Statut:
ppublish
Résumé
With the renewed enthusiasm in immuno-oncology, characterization of the tumor immune microenvironment constitutes an essential and unique aspect to the assessment of therapeutics. The isolation of tumor-infiltrating lymphocytes (TILs) is a desirable approach toward the understanding of antitumor immune response. This chapter provides an effective protocol to mechanically dissociate tumor tissue and generate single-cell suspension from excised tumors. TILs are then isolated by Ficoll-Paque density gradient centrifugation. This protocol is applicable to both human and experimental tumors in immunocompetent murine models.
Identifiants
pubmed: 30798524
doi: 10.1007/978-1-4939-9167-9_8
pmc: PMC6533618
mid: NIHMS1022419
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
93-99Subventions
Organisme : NIDCR NIH HHS
ID : K99 DE024173
Pays : United States
Organisme : NIDCR NIH HHS
ID : R01 DE026728
Pays : United States
Organisme : NIDCR NIH HHS
ID : R03 DE027399
Pays : United States
Organisme : NIDCR NIH HHS
ID : R00 DE024173
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI007413
Pays : United States
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