Assessing Acinetobacter baumannii Virulence and Persistence in a Murine Model of Lung Infection.


Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2019
Historique:
entrez: 25 2 2019
pubmed: 25 2 2019
medline: 23 7 2019
Statut: ppublish

Résumé

Acinetobacter baumannii is a Gram-negative opportunistic pathogen and a leading cause of ventilator-associated pneumonia. Murine models of A. baumannii lung infection allow researchers to experimentally assess A. baumannii virulence and host response. Intranasal administration of A. baumannii models acute lung infection. This chapter describes the methods to test A. baumannii virulence in a murine model of lung infection, including assessing the competitive index of a bacterial mutant and the associated inflammatory responses.

Identifiants

pubmed: 30798564
doi: 10.1007/978-1-4939-9118-1_26
pmc: PMC6446551
mid: NIHMS1016173
doi:

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

289-305

Subventions

Organisme : NIAID NIH HHS
ID : F32 AI122516
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI101171
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM008320
Pays : United States
Organisme : NHLBI NIH HHS
ID : T32 HL094296
Pays : United States

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Auteurs

Lauren D Palmer (LD)

Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA.
Vanderbilt Institute for Infection, Immunology and Inflammation, Vanderbilt University Medical Center, Nashville, TN, USA.

Erin R Green (ER)

Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA.
Vanderbilt Institute for Infection, Immunology and Inflammation, Vanderbilt University Medical Center, Nashville, TN, USA.

Jessica R Sheldon (JR)

Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA.
Vanderbilt Institute for Infection, Immunology and Inflammation, Vanderbilt University Medical Center, Nashville, TN, USA.

Eric P Skaar (EP)

Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA. eric.skaar@vumc.org.
Vanderbilt Institute for Infection, Immunology and Inflammation, Vanderbilt University Medical Center, Nashville, TN, USA. eric.skaar@vumc.org.

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Classifications MeSH