Formation of Large Scaffold-Free 3-D Aggregates in a Cell Culture Dish by Ultrasound Standing Wave Trapping.

Animals Cell Aggregation / physiology Cell Culture Techniques / methods Mice Models, Animal Myoblasts / physiology Tissue Engineering / methods Ultrasonic Waves
Mouse myoblasts Scaffold-free aggregates Three-dimensional aggregates Tissue engineering Ultrasound standing wave trapping Ultrasound transducer

Journal

Ultrasound in medicine & biology
ISSN: 1879-291X
Titre abrégé: Ultrasound Med Biol
Pays: England
ID NLM: 0410553

Informations de publication

Date de publication:
05 2019
Historique:
received: 10 03 2018
revised: 25 12 2018
accepted: 15 01 2019
pubmed: 26 2 2019
medline: 26 12 2019
entrez: 26 2 2019
Statut: ppublish

Résumé

Cellular aggregates that mimic cell-cell interactions in vitro are essential for biological research. This study introduces a method to form large scaffold-free 3-D aggregates in a clinically ubiquitous cell culture dish using kilohertz-order ultrasound standing wave trapping (USWT). We fabricated an aggregate formation system in which a 60-mm dish was set above a Langevin transducer via water. The transducer was excited at 110.8 kHz, and then C2C12 myoblasts were injected into the dish and trapped at the node position of the standing wave. The diameter and thickness of the formed aggregate were 8 and 2.7 mm, respectively, which are larger than those of aggregates formed previously by USWT. Moreover, we confirmed that >94% of cells constituting the aggregates survived 9 h, and the protein expression of cells was not altered significantly. This method can be applied to form aggregates with high functionality, which contributes to the development of biological research methodology.

Identifiants

DOI: 10.1016/j.ultrasmedbio.2019.01.013 PMID: 30799124
pubmed: 30799124
pii: S0301-5629(19)30022-5
doi: 10.1016/j.ultrasmedbio.2019.01.013
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1306-1315

Informations de copyright

Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.

Auteurs

Misa Nakao (M)

School of Science for Open and Environmental Systems, Graduate School of Science and Technology, Keio University, Yokohama, Kanagawa, Japan.

Chikahiro Imashiro (C)

School of Science for Open and Environmental Systems, Graduate School of Science and Technology, Keio University, Yokohama, Kanagawa, Japan.

Taiki Kuribara (T)

Department of Materials and Life Science, Faculty of Science and Technology, Seikei University, Musashino, Tokyo, Japan.

Yuta Kurashina (Y)

Department of Materials Science and Engineering, School of Materials and Chemical Technology, Tokyo Institute of Technology, Yokohama, Kanagawa, Japan 226-8503; Department of Mechanical Engineering, Faculty of Science and Technology, Keio University, Yokohama, Kanagawa, Japan.

Kiichiro Totani (K)

Department of Materials and Life Science, Faculty of Science and Technology, Seikei University, Musashino, Tokyo, Japan.

Kenjiro Takemura (K)

Department of Mechanical Engineering, Faculty of Science and Technology, Keio University, Yokohama, Kanagawa, Japan. Electronic address: takemura@mech.keio.ac.jp.

Articles similaires

Evaluating the efficacy of telesurgery with dual console SSI Mantra Surgical Robotic System: experiment on animal model and clinical trials.

Sudhir Prem Srivastava, Vishwajyoti Pascual Srivastava, Avinesh Singh et al.
1.00
Robotic Surgical Procedures Animals Humans Telemedicine Models, Animal

Odour generalisation and detection dog training.

Lyn Caldicott, Thomas W Pike, Helen E Zulch et al.
1.00
Animals Odorants Dogs Generalization, Psychological Smell

FBXO22 inhibits colitis and colorectal carcinogenesis by regulating the degradation of the S2448-phosphorylated form of mTOR.

Minle Li, Xuan Chen, Pengfei Qu et al.
1.00
Animals TOR Serine-Threonine Kinases Colorectal Neoplasms Colitis Mice

Use of organic material provided by an automatic enrichment device by weaner pigs and its influence on tail lesions.

Philipp Heseker, Jeanette Probst, Stefanie Ammer et al.
1.00
Animals Tail Swine Behavior, Animal Animal Husbandry

Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Formation of Large Scaffold-Free 3-D...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Agrégation cellulaire › Formation of Large Scaffold-Free 3-D...
questionsmedicales.fr › Techniques d'investigation › Techniques in vitro › Techniques de culture › Techniques de culture cellulaire › Formation of Large Scaffold-Free 3-D...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Formation of Large Scaffold-Free 3-D...
questionsmedicales.fr › Techniques d'investigation › Modèles animaux › Formation of Large Scaffold-Free 3-D...
questionsmedicales.fr › Cellules › Cellules souches › Myoblastes › Formation of Large Scaffold-Free 3-D...
questionsmedicales.fr › Technologie, industrie et agriculture › Ingénierie › Bioingénierie › Ingénierie cellulaire › Ingénierie tissulaire › Formation of Large Scaffold-Free 3-D...
questionsmedicales.fr › Phénomènes physiques › Rayonnement › Rayonnement non ionisant › Son (physique) › Ondes ultrasonores › Formation of Large Scaffold-Free 3-D...