Glypican-1 and glycoprotein 2 bearing extracellular vesicles do not discern pancreatic cancer from benign pancreatic diseases.
extracellular vesicles
flow cytometry
glypican-1
liquid biopsy
pancreatic cancer
Journal
Oncotarget
ISSN: 1949-2553
Titre abrégé: Oncotarget
Pays: United States
ID NLM: 101532965
Informations de publication
Date de publication:
01 Feb 2019
01 Feb 2019
Historique:
received:
04
12
2018
accepted:
09
01
2019
entrez:
26
2
2019
pubmed:
26
2
2019
medline:
26
2
2019
Statut:
epublish
Résumé
Pancreatic ductal adenocarcinoma (PDAC) is a lethal disease that is clinically asymptomatic in its early stages of development. Non-invasive testing for pancreatic cancer biomarkers would significantly improve early detection and patient care. Extracellular vesicles (EVs) are circulating tumor fragments present in the blood and may express cancer specific biomarkers that would enable early detection of pancreatic cancer. We tested the utility of a blood test enumerating EVs positive for the pancreas-specific marker Glycoprotein 2 (GP2) and the putative pancreatic cancer marker Glypican-1 (GPC1) in patients with PDAC. Various levels of GPC1-positive and GP2/GPC1-positive EVs were detected in PDAC patients but were not significantly higher than benign pancreatic disease (BPD) patients. The sensitivity and specificity of the GPC1 EV test was 26.67% and 87.50% respectively, whereas the sensitivity and specificity for the GPC1+GP2 EV test was 23.33% and 90.00% respectively. Immunohistochemistry of GPC1 expression in a tissue microarray of PDAC and various controls also did not demonstrate specificity of GPC1 to PDAC. Hence, enumeration of GPC1-positive EVs, solely or in conjunction with GP2, was unable to effectively distinguish between BPD and pancreatic cancer.
Identifiants
pubmed: 30800217
doi: 10.18632/oncotarget.26620
pii: 26620
pmc: PMC6383691
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1045-1055Déclaration de conflit d'intérêts
CONFLICTS OF INTEREST The authors declare no conflicts of interest.
Références
Blood. 1999 Dec 1;94(11):3791-9
pubmed: 10572093
Cancer Res. 2001 Jul 15;61(14):5562-9
pubmed: 11454708
Arch Surg. 2003 Apr;138(4):427-3; discussion 433-4
pubmed: 12686529
J Gastroenterol Hepatol. 2004 Feb;19(2):182-6
pubmed: 14731128
Dis Colon Rectum. 2004 Feb;47(2):227-32
pubmed: 15043294
Lancet. 2004 Mar 27;363(9414):1049-57
pubmed: 15051286
Arch Pathol Lab Med. 2004 Jun;128(6):668-74
pubmed: 15163232
Clin Gastroenterol Hepatol. 2004 Jun;2(6):510-7
pubmed: 15181621
Eur J Surg Oncol. 2005 Mar;31(2):164-9
pubmed: 15698733
Leukemia. 2006 May;20(5):847-56
pubmed: 16453000
Am J Pathol. 2006 Jun;168(6):2014-26
pubmed: 16723715
Cancer Cell. 2008 Mar;13(3):221-34
pubmed: 18328426
Gynecol Oncol. 2008 Jul;110(1):13-21
pubmed: 18589210
Clin Lung Cancer. 2009 Jan;10(1):42-6
pubmed: 19289371
Eur J Cell Biol. 1990 Oct;53(1):154-63
pubmed: 2076702
Semin Immunopathol. 2011 Sep;33(5):455-67
pubmed: 21318413
Lancet. 2011 Aug 13;378(9791):607-20
pubmed: 21620466
Nucleic Acids Res. 2012 Jan;40(Database issue):D1241-4
pubmed: 21989406
J Gastrointest Surg. 2012 Apr;16(4):771-83
pubmed: 22127781
PLoS One. 2012;7(1):e30177
pubmed: 22276156
Bioessays. 2012 Jun;34(6):489-97
pubmed: 22442051
PLoS One. 2012;7(4):e34653
pubmed: 22506041
Asian J Surg. 2013 Jan;36(1):7-12
pubmed: 23270819
J Neurooncol. 2013 May;113(1):1-11
pubmed: 23456661
Cancer Res. 2013 Nov 1;73(21):6384-8
pubmed: 24145355
Biochem Biophys Res Commun. 1988 Jul 29;154(2):818-23
pubmed: 2456764
Nat Biotechnol. 2014 May;32(5):441-3
pubmed: 24811515
Nature. 2015 Jul 9;523(7559):177-82
pubmed: 26106858
Oncotarget. 2016 Feb 23;7(8):8839-49
pubmed: 26814433
Nature. 2016 Mar 3;531(7592):47-52
pubmed: 26909576
Cancer Lett. 2017 May 1;393:86-93
pubmed: 28232049
Sci Transl Med. 2017 May 24;9(391):
pubmed: 28539469
Front Cell Dev Biol. 2017 Aug 30;5:78
pubmed: 28913335
ACS Nano. 2017 Nov 28;11(11):11182-11193
pubmed: 29019651
Methods Mol Biol. 2018;1678:79-92
pubmed: 29071676
Oncotarget. 2018 Apr 10;9(27):19006-19013
pubmed: 29721179
Thromb Haemost. 2018 Sep;118(9):1612-1624
pubmed: 30112749