Validation of clinic-based cryptococcal antigen lateral flow assay screening in HIV-infected adults in South Africa.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
25 02 2019
Historique:
received: 04 09 2018
accepted: 06 12 2018
entrez: 27 2 2019
pubmed: 26 2 2019
medline: 15 9 2020
Statut: epublish

Résumé

Since rapid cryptococcal antigen lateral flow assays (CrAg LFA) may expedite treatment of HIV-associated cryptococcal infections, we sought to validate clinic-based CrAg LFA testing. Among newly-diagnosed HIV-infected adults in South Africa, a trained nurse performed clinic-based testing of urine, fingerprick capillary and venous whole blood with rapid CrAg LFA (Immy Diagnostics, Norman, USA). We performed matched laboratory-based serum cryptococcal antigen testing with an enzyme immunoassay (EIA). We assessed diagnostic accuracy using EIA as the gold-standard, and performed additional validation testing on serum and among hospitalized adults with cryptococcal meningitis. Among 5,618 participants enrolled, 1,296 were HIV-infected and screened for cryptococcal antigenemia. Overall CrAg prevalence by serum EIA was 3.6% (95% CI 2.0-6.0%) for adults with CD4 < 200 cells/mm

Identifiants

pubmed: 30804356
doi: 10.1038/s41598-018-37478-7
pii: 10.1038/s41598-018-37478-7
pmc: PMC6389876
doi:

Substances chimiques

Antigens, Fungal 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

2687

Subventions

Organisme : NIAID NIH HHS
ID : K23 AI108293
Pays : United States
Organisme : NIAID NIH HHS
ID : P30 AI060354
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI110264
Pays : United States

Références

AIDS. 2002 May 3;16(7):1031-8
pubmed: 11953469
Trop Med Int Health. 2007 Aug;12(8):929-35
pubmed: 17697087
Clin Infect Dis. 2009 Apr 1;48(7):856-62
pubmed: 19222372
BMC Infect Dis. 2010 Mar 15;10:67
pubmed: 20230635
Clin Infect Dis. 2011 Nov;53(10):1019-23
pubmed: 21940419
J Acquir Immune Defic Syndr. 2012 Apr 15;59(5):e85-91
pubmed: 22410867
Clin Vaccine Immunol. 2013 Jan;20(1):52-5
pubmed: 23114703
PLoS One. 2012;7(11):e49541
pubmed: 23166705
MLO Med Lab Obs. 2013 Mar;45(3):16, 18, 20
pubmed: 23822028
Trop Med Int Health. 2013 Sep;18(9):1075-1079
pubmed: 23937699
PLoS One. 2013 Oct 04;8(10):e75585
pubmed: 24124498
J Int AIDS Soc. 2014 Aug 08;17:19040
pubmed: 25109284
Lancet. 2015 May 30;385(9983):2173-82
pubmed: 25765698
Clin Infect Dis. 2015 Aug 1;61(3):464-7
pubmed: 25838287
Open Forum Infect Dis. 2015 Apr 02;2(2):ofv046
pubmed: 26213690
AIDS. 2015 Nov 28;29(18):2473-8
pubmed: 26372487
Clin Infect Dis. 2016 Mar 1;62(5):581-587
pubmed: 26565007
BMJ Open. 2016 Nov 14;6(11):e012799
pubmed: 28137831
Lancet Infect Dis. 2017 Aug;17(8):873-881
pubmed: 28483415
Clin Infect Dis. 2018 Feb 10;66(5):686-692
pubmed: 29028998
J Clin Microbiol. 2018 Mar 26;56(4):
pubmed: 29367291
Clin Infect Dis. 2018 Mar 4;66(suppl_2):S152-S159
pubmed: 29514236
Front Microbiol. 2018 Mar 13;9:409
pubmed: 29593675

Auteurs

Paul K Drain (PK)

Departments of Global Health, University of Washington, Seattle, USA. pkdrain@uw.edu.
Medicine, University of Washington, Seattle, USA. pkdrain@uw.edu.
Epidemiology, University of Washington, Seattle, USA. pkdrain@uw.edu.

Ting Hong (T)

Departments of Global Health, University of Washington, Seattle, USA.

Meighan Krows (M)

Departments of Global Health, University of Washington, Seattle, USA.

Sabina Govere (S)

AIDS Healthcare Foundation, Durban, South Africa.

Hilary Thulare (H)

AIDS Healthcare Foundation, Durban, South Africa.

Carole L Wallis (CL)

BARC-SA and Lancet Laboratory, Johannesburg, South Africa.

Bernadett I Gosnell (BI)

Department of Infectious Diseases, University of KwaZulu-Natal, Durban, South Africa.

Mahomed-Yunus Moosa (MY)

Department of Infectious Diseases, University of KwaZulu-Natal, Durban, South Africa.

Ingrid V Bassett (IV)

Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, USA.

Connie Celum (C)

Departments of Global Health, University of Washington, Seattle, USA.
Medicine, University of Washington, Seattle, USA.

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female
Humans United States Aged Cross-Sectional Studies Medicare Part C
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH