Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation.
Cell biology*
Cellular organelles*
Endocytosis*
Immunology*
Inflammatory response
Label-free quantification
Protein Identification*
dendritic cell
phagocytosis
phagosome maturation
Journal
Molecular & cellular proteomics : MCP
ISSN: 1535-9484
Titre abrégé: Mol Cell Proteomics
Pays: United States
ID NLM: 101125647
Informations de publication
Date de publication:
05 2019
05 2019
Historique:
received:
10
01
2019
revised:
23
02
2019
pubmed:
28
2
2019
medline:
17
1
2020
entrez:
28
2
2019
Statut:
ppublish
Résumé
Dendritic cells (DCs) are professional phagocytes that use innate sensing and phagocytosis to internalize and degrade self as well as foreign material, such as pathogenic bacteria, within phagosomes. These intracellular compartments are equipped to generate antigenic peptides that serve as source for antigen presentation to T cells initiating adaptive immune responses. The phagosomal proteome of DCs is only partially studied and is highly dynamic as it changes during phagosome maturation, when phagosomes sequentially interact with endosomes and lysosomes. In addition, the activation status of the phagocyte can modulate the phagosomal composition and is able to shape phagosomal functions.In this study, we determined spatiotemporal changes of the proteome of DC phagosomes during their maturation and compared resting and lipopolysaccharide (LPS)-stimulated bone marrow-derived DCs by label-free, quantitative mass spectrometry. Ovalbumin-coupled latex beads were used as phagocytosis model system and revealed that LPS-treated DCs show decreased recruitment of proteins involved in phagosome maturation, such as subunits of the vacuolar proton ATPase, cathepsin B, D, S, and RAB7. In contrast, those phagosomes were characterized by an increased recruitment of proteins involved in antigen cross-presentation,
Identifiants
pubmed: 30808727
pii: S1535-9476(20)31602-9
doi: 10.1074/mcp.RA119.001316
pmc: PMC6495253
pii:
doi:
Substances chimiques
Lipopolysaccharides
0
Proteome
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
909-922Subventions
Organisme : Medical Research Council
ID : MC_UP_A500_1020
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UU_12016/5
Pays : United Kingdom
Informations de copyright
© 2019 Pauwels et al.
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