Peripheral-blood b-cell subset disturbances in inflammatory joint diseases induced by Tropheryma whipplei.
Adolescent
Adult
Aged
Aged, 80 and over
Anti-Bacterial Agents
/ administration & dosage
B-Lymphocyte Subsets
/ immunology
Ceftriaxone
/ administration & dosage
Doxycycline
/ therapeutic use
Female
Flow Cytometry
Humans
Hydroxychloroquine
/ therapeutic use
Lymphocyte Activation
Male
Middle Aged
ROC Curve
Retrospective Studies
Tropheryma
Whipple Disease
/ drug therapy
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2019
2019
Historique:
received:
17
08
2018
accepted:
16
01
2019
entrez:
28
2
2019
pubmed:
28
2
2019
medline:
4
12
2019
Statut:
epublish
Résumé
To look for abnormalities in circulating B-cell subsets in patients with rheumatic symptoms of Whipple's disease (WD). Consecutive patients seen between 2010 and 2016 for suspected inflammatory joint disease were identified retrospectively. Results of standardized immunological and serological tests and of peripheral-blood B-cell and T-cell subset analysis by flow cytometry were collected. Patients with criteria suggesting WD underwent PCR testing for Tropheryma whipplei, and those with diagnosis of WD (cases) were compared to those without diagnosis (controls). We used ROC curve analysis to evaluate the diagnostic value of flow cytometry findings for WD. Among 2917 patients seen for suspected inflammatory joint disease, 121 had suspected WD, including 9 (9/121, 7.4%) confirmed WD. Proportions of T cells and NK cells were similar between suspected and confirmed WD, whereas cases had a lower proportion of circulating memory B cells (IgD-CD38low, 18.0%±9.7% vs. 26.0%±14.2%, P = 0.041) and higher ratio of activated B cells over memory B cells (4.4±2.0 vs. 2.9±2.2, P = 0.023). Among peripheral-blood B-cells, the proportion of IgD+CD27- naive B cells was higher (66.2%±18.2% vs. 54.6%±18.4%, P = 0.047) and that of IgD-CD27+ switched memory B cells lower (13.3%±5.7% vs. 21.4%±11.9%, P = 0.023), in cases vs. controls. The criterion with the best diagnostic performance was a proportion of IgD+CD27- naive B cells above 70.5%, which had 73% sensitivity and 80% specificity. Our study provides data on peripheral-blood B-cell disturbances that may have implications for the diagnosis and pathogenetic understanding of WD.
Identifiants
pubmed: 30811404
doi: 10.1371/journal.pone.0211536
pii: PONE-D-18-24321
pmc: PMC6392227
doi:
Substances chimiques
Anti-Bacterial Agents
0
Hydroxychloroquine
4QWG6N8QKH
Ceftriaxone
75J73V1629
Doxycycline
N12000U13O
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0211536Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Gastroenterology. 2010 Jan;138(1):210-20
pubmed: 19664628
Lancet. 1991 Aug 24;338(8765):474-5
pubmed: 1714530
J Infect Dis. 2008 Mar 15;197(6):880-7
pubmed: 18419351
Autoimmun Rev. 2016 Sep;15(9):883-9
pubmed: 27392502
Eur J Clin Microbiol Infect Dis. 2008 Oct;27(10):959-68
pubmed: 18594884
Gastroenterology. 2009 Jun;136(7):2289-94
pubmed: 19208355
Int J Syst Evol Microbiol. 2001 Jul;51(Pt 4):1471-9
pubmed: 11491348
Rheumatology (Oxford). 2017 Aug 1;56(8):1401-1406
pubmed: 28431111
Expert Rev Anti Infect Ther. 2012 Mar;10(3):253-5
pubmed: 22397556
Aliment Pharmacol Ther. 2015 Apr;41(8):709-24
pubmed: 25693648
Clin Infect Dis. 2008 Sep 1;47(5):659-67
pubmed: 18662136
Lancet Infect Dis. 2008 Mar;8(3):179-90
pubmed: 18291339
Acta Clin Belg. 2016 Dec;71(6):373-378
pubmed: 27884091
J Neurol Sci. 2017 Jun 15;377:197-206
pubmed: 28477696
Future Microbiol. 2017 Mar;12:247-254
pubmed: 28262041
J Infect. 2014 Aug;69(2):103-12
pubmed: 24877762
Infection. 2003 Mar;31(2):81-5
pubmed: 12682812
Gastroenterology. 2002 Nov;123(5):1468-77
pubmed: 12404221
J Antimicrob Chemother. 2014 Jan;69(1):219-27
pubmed: 23946319
J Infect Dis. 2005 Nov 1;192(9):1642-6
pubmed: 16206080
Medicine (Baltimore). 2010 Sep;89(5):337-45
pubmed: 20827111
Arthritis Rheumatol. 2014 Sep;66(9):2590-600
pubmed: 24909567
Int J Colorectal Dis. 2006 Mar;21(2):114-20
pubmed: 15875203
N Engl J Med. 1992 Jul 30;327(5):293-301
pubmed: 1377787
Ann Rheum Dis. 2009 Sep;68(9):1447-52
pubmed: 18782791
Arthritis Rheum. 2007 May;56(5):1713-8
pubmed: 17469186
J Clin Microbiol. 2012 Dec;50(12):3917-20
pubmed: 23015670
Lancet Infect Dis. 2016 Mar;16(3):e13-22
pubmed: 26856775
J Immunol. 2008 Jan 15;180(2):688-92
pubmed: 18178805
Clin Rev Allergy Immunol. 2017 Oct;53(2):219-236
pubmed: 28474288
J Immunol. 2006 Aug 1;177(3):2015-22
pubmed: 16849516
Medicine (Baltimore). 2015 Apr;94(15):e714
pubmed: 25881849
Future Microbiol. 2013 Jan;8(1):57-71
pubmed: 23252493
Ann Intern Med. 2010 Dec 7;153(11):710-7
pubmed: 21135294
Arthritis Rheum. 1999 Apr;42(4):812-7
pubmed: 10211899
J Infect Dis. 2009 Apr 1;199(7):1043-52
pubmed: 19222368
Ann Rheum Dis. 2002 Jun;61(6):554-8
pubmed: 12006334
Gastroenterology. 1997 Aug;113(2):442-8
pubmed: 9247462
PLoS One. 2018 Jul 18;13(7):e0200645
pubmed: 30020975
Expert Rev Mol Diagn. 2001 Sep;1(3):299-309
pubmed: 11901835
N Engl J Med. 2007 Jan 4;356(1):55-66
pubmed: 17202456
Elife. 2018 Mar 14;7:
pubmed: 29537367
Am J Respir Crit Care Med. 2016 Jul 15;194(2):243-5
pubmed: 27420361
J Clin Microbiol. 2002 Mar;40(3):1119-20
pubmed: 11880458