Therapeutic Effect of the Quinolone Levofloxacin on Inflammatory Fibroblast-like Synoviocytes.
Animals
Apoptosis
Cell Proliferation
Cells, Cultured
Fibroblasts
/ drug effects
Inflammation
/ drug therapy
Levofloxacin
/ pharmacology
Matrix Metalloproteinase 1
/ metabolism
Matrix Metalloproteinase 3
/ metabolism
Rabbits
Synoviocytes
/ drug effects
Tissue Inhibitor of Metalloproteinase-1
/ metabolism
Topoisomerase II Inhibitors
/ pharmacology
apoptosis
fibroblast-like synoviocyte
inflammatory
levofloxacin
proliferation
Journal
Annals of clinical and laboratory science
ISSN: 1550-8080
Titre abrégé: Ann Clin Lab Sci
Pays: United States
ID NLM: 0410247
Informations de publication
Date de publication:
Jan 2019
Jan 2019
Historique:
entrez:
1
3
2019
pubmed:
1
3
2019
medline:
17
7
2019
Statut:
ppublish
Résumé
This study aims to analyze the effects of quinolones on synovium. The present study explored the effects of levofloxacin on fibroblast-like synoviocyte (FLS) When treated with levofloxacin for 24 hours, cell viability decreased in a concentration-dependent manner. Furthermore, the protein expression level of active caspase-3 increased, while the protein expression level of MMP-3 revealed no significant changes. In addition, the mRNA levels of MMP-1, MMP-3 and TIMP-1 all increased. Moreover, the mRNA levels of IL-6 and COX-2 decreased, while the mRNA levels of Caspase-3 and -8 increased, both in a concentration-dependent manner. The anti-inflammatory and anti-proliferative effects of levofloxacin on FLS are mediated by inhibiting inflammatory cytokines and inducing apoptosis in rabbits. Thus, quinolones might possess a special effect to treat synovitis and relieve inflammatory joint disease.
Substances chimiques
TIMP1 protein, human
0
Tissue Inhibitor of Metalloproteinase-1
0
Topoisomerase II Inhibitors
0
Levofloxacin
6GNT3Y5LMF
MMP3 protein, human
EC 3.4.24.17
Matrix Metalloproteinase 3
EC 3.4.24.17
MMP1 protein, human
EC 3.4.24.7
Matrix Metalloproteinase 1
EC 3.4.24.7
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
9-15Informations de copyright
© 2019 by the Association of Clinical Scientists, Inc.