Inhibition of Candida albicans morphogenesis by chitinase from Lactobacillus rhamnosus GG.
Bacterial Proteins
/ metabolism
Biological Therapy
Candida albicans
/ pathogenicity
Candidiasis
/ therapy
Chitin
/ metabolism
Chitinases
/ metabolism
Humans
Hyphae
/ physiology
Lacticaseibacillus rhamnosus
/ physiology
Morphogenesis
Probiotics
/ therapeutic use
RNA Interference
RNA, Ribosomal, 16S
/ genetics
Species Specificity
Virulence
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
27 02 2019
27 02 2019
Historique:
received:
22
11
2018
accepted:
24
01
2019
entrez:
1
3
2019
pubmed:
1
3
2019
medline:
24
10
2020
Statut:
epublish
Résumé
Lactobacilli have been evaluated as probiotics against Candida infections in several clinical trials, but with variable results. Predicting and understanding the clinical efficacy of Lactobacillus strains is hampered by an overall lack of insights into their modes of action. In this study, we aimed to unravel molecular mechanisms underlying the inhibitory effects of lactobacilli on hyphal morphogenesis, which is a crucial step in C. albicans virulence. Based on a screening of different Lactobacillus strains, we found that the closely related taxa L. rhamnosus, L. casei and L. paracasei showed stronger activity against Candida hyphae formation compared to other Lactobacillus species tested. By exploring the activity of purified compounds and mutants of the model strain L. rhamnosus GG, the major peptidoglycan hydrolase Msp1, conserved in the three closely related taxa, was identified as a key effector molecule. We could show that this activity of Msp1 was due to its ability to break down chitin, the main polymer in the hyphal cell wall of C. albicans. This identification of a Lactobacillus-specific protein with chitinase activity having anti-hyphal activity will assist in better strain selection and improved application in future clinical trials for Lactobacillus-based Candida-management strategies.
Identifiants
pubmed: 30814593
doi: 10.1038/s41598-019-39625-0
pii: 10.1038/s41598-019-39625-0
pmc: PMC6393446
doi:
Substances chimiques
Bacterial Proteins
0
RNA, Ribosomal, 16S
0
Chitin
1398-61-4
Chitinases
EC 3.2.1.14
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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