Detection of Mycobacterium leprae DNA in soil: multiple needles in the haystack.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
28 02 2019
28 02 2019
Historique:
received:
22
08
2018
accepted:
25
01
2019
entrez:
1
3
2019
pubmed:
1
3
2019
medline:
17
9
2020
Statut:
epublish
Résumé
Leprosy is an infectious disease caused by Mycobacterium leprae affecting the skin and nerves. Despite decades of availability of adequate treatment, transmission is unabated and transmission routes are not completely understood. Despite the general assumption that untreated M. leprae infected humans represent the major source of transmission, scarce reports indicate that environmental sources could also play a role as a reservoir. We investigated whether M. leprae DNA is present in soil of regions where leprosy is endemic or areas with possible animal reservoirs (armadillos and red squirrels). Soil samples (n = 73) were collected in Bangladesh, Suriname and the British Isles. Presence of M. leprae DNA was determined by RLEP PCR and genotypes were further identified by Sanger sequencing. M. leprae DNA was identified in 16.0% of soil from houses of leprosy patients (Bangladesh), in 10.7% from armadillos' holes (Suriname) and in 5% from the habitat of lepromatous red squirrels (British Isles). Genotype 1 was found in Bangladesh whilst in Suriname the genotype was 1 or 2. M. leprae DNA can be detected in soil near human and animal sources, suggesting that environmental sources represent (temporary) reservoirs for M. leprae.
Identifiants
pubmed: 30816338
doi: 10.1038/s41598-019-39746-6
pii: 10.1038/s41598-019-39746-6
pmc: PMC6395756
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3165Références
J Med Microbiol. 2001 Feb;50(2):177-182
pubmed: 11211226
Clin Infect Dis. 2016 Dec 1;63(11):1412-1420
pubmed: 27558564
Nat Commun. 2018 Jan 24;9(1):352
pubmed: 29367657
Lepr Rev. 2015 Jun;86(2):142-55
pubmed: 26502685
Indian J Med Microbiol. 2016 Jul-Sep;34(3):315-21
pubmed: 27514953
Int J Mycobacteriol. 2015 Mar;4(1):54-9
pubmed: 26655199
PLoS Negl Trop Dis. 2018 May 25;12(5):e0006529
pubmed: 29799844
Infect Genet Evol. 2008 Sep;8(5):627-31
pubmed: 18599381
PLoS Negl Trop Dis. 2018 Jan 30;12(1):e0006190
pubmed: 29381722
Emerg Infect Dis. 2018 Jan;24(1):165-166
pubmed: 29260666
PLoS Negl Trop Dis. 2018 Jun 28;12(6):e0006532
pubmed: 29953440
Infect Genet Evol. 2012 Jan;12(1):121-6
pubmed: 22101333
Lepr Rev. 2001 Dec;72(4):449-61
pubmed: 11826481
Rev Soc Bras Med Trop. 2017 Mar-Apr;50(2):216-222
pubmed: 28562758
J Commun Dis. 2006 Mar;38(3):269-73
pubmed: 17373359
Science. 2005 May 13;308(5724):1040-2
pubmed: 15894530
EBioMedicine. 2017 Sep;23:119-124
pubmed: 28882756
Appl Environ Microbiol. 1992 Jul;58(7):2292-5
pubmed: 1386212
Mem Inst Oswaldo Cruz. 2017 Dec;112(12):805-811
pubmed: 29211240
Lepr Rev. 1995 Dec;66(4):287-95
pubmed: 8637382
Nat Genet. 2009 Dec;41(12):1282-9
pubmed: 19881526
PLoS Negl Trop Dis. 2011 May 10;5(5):e1029
pubmed: 21572979
J Clin Microbiol. 2018 Feb 22;56(3):
pubmed: 29305545
Vet Rec. 2015 Aug 22;177(8):206-207
pubmed: 28319925
J Clin Microbiol. 2015 Jun;53(6):1945-6
pubmed: 25809978
Lepr Rev. 2004 Dec;75(4):310-26
pubmed: 15682969
PLoS Negl Trop Dis. 2014 Dec 18;8(12):e3405
pubmed: 25521850
Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4459-64
pubmed: 25831531
Indian J Dermatol Venereol Leprol. 2016 Jan-Feb;82(1):23-7
pubmed: 26728806
Mamm Genome. 2011 Feb;22(1-2):19-31
pubmed: 20936290
Wkly Epidemiol Rec. ;92(35):501-19
pubmed: 28861986
Science. 2016 Nov 11;354(6313):744-747
pubmed: 27846605
N Engl J Med. 2011 Apr 28;364(17):1626-33
pubmed: 21524213
Clin Microbiol Infect. 2014 Mar;20(3):O142-9
pubmed: 24520878
Can J Microbiol. 2000 Jul;46(7):633-42
pubmed: 10932357
Nat Genet. 2015 Mar;47(3):267-71
pubmed: 25642632