Self-crosslinked fibrous collagen/chitosan blends: Processing, properties evaluation and monitoring of degradation by bi-fluorescence imaging.

Animals Biocompatible Materials / chemistry Cell Survival Chemical Phenomena Chitosan / chemistry Collagen / chemistry Materials Testing Mechanical Phenomena Mice Optical Imaging / methods Spectroscopy, Fourier Transform Infrared Thermogravimetry Tissue Scaffolds / chemistry
Bi-fluorescence imaging Collagen:chitosan scaffolds Dehydrothermal treatment

Journal

International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578

Informations de publication

Date de publication:
15 Jun 2019
Historique:
received: 10 01 2019
revised: 22 02 2019
accepted: 22 02 2019
pubmed: 1 3 2019
medline: 10 9 2019
entrez: 1 3 2019
Statut: ppublish

Résumé

Porous collagen/chitosan scaffolds with different Collagen:Chitosan (Coll:Ch) ratios were prepared by freeze-drying followed by self-crosslinking via dehydrothermal treatment (DHT) and characterized as biomaterials for tissue engineering. Cy7 and Cy5.5 fluorochromes were covalently grafted to collagen and chitosan, respectively. Thus, it was possible, using optical fluorescence imaging of the two fluorochromes, to simultaneously track their in vivo biodegradation, in a blend scaffold form. The fluorescence signal evolution, due to the bioresorption, corroborated with histological analysis. In vitro cytocompatibility of Coll:Ch blend scaffolds were evaluated with standardized tests. In addition, the scaffolds showed a highly interconnected porous structure. Extent of crosslinking was analyzed by convergent analysis using thermogravimetry, Fourier Transform Infrared Spectroscopy and PBS uptake. The variations observed with these techniques indicate strong interactions between collagen and chitosan (covalent and hydrogen bonds) promoted by the DHT. The mechanical properties were characterized to elucidate the impact of the different processing steps in the sample preparation (DHT, neutralization and sterilization by β-irradiation) and showed a robust processing scheme with low impact of Coll:Ch composition ratio.

Identifiants

DOI: 10.1016/j.ijbiomac.2019.02.134 PMID: 30817967
pubmed: 30817967
pii: S0141-8130(19)30244-2
doi: 10.1016/j.ijbiomac.2019.02.134
pii:
doi:

Substances chimiques

Biocompatible Materials 0
Collagen 9007-34-5
Chitosan 9012-76-4

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

353-367

Informations de copyright

Copyright © 2019 Elsevier B.V. All rights reserved.

Auteurs

P N Oliveira (PN)

IMP, CNRS UMR 5223, Univ Claude Bernard Lyon 1, Univ Lyon, 15 bd Latarjet, 69622 Villeurbanne, France. Electronic address: paulaoliveiraqmc@gmail.com.

A Montembault (A)

IMP, CNRS UMR 5223, Univ Claude Bernard Lyon 1, Univ Lyon, 15 bd Latarjet, 69622 Villeurbanne, France.

G Sudre (G)

IMP, CNRS UMR 5223, Univ Claude Bernard Lyon 1, Univ Lyon, 15 bd Latarjet, 69622 Villeurbanne, France.

P Alcouffe (P)

IMP, CNRS UMR 5223, Univ Claude Bernard Lyon 1, Univ Lyon, 15 bd Latarjet, 69622 Villeurbanne, France.

L Marcon (L)

ILM, CNRS UMR 5306, Univ Claude Bernard Lyon 1, Univ Lyon, 43 bd du 11 Nov 1918, 69622 Villeurbanne, France.

H Gehan (H)

ILM, CNRS UMR 5306, Univ Claude Bernard Lyon 1, Univ Lyon, 43 bd du 11 Nov 1918, 69622 Villeurbanne, France.

F Lux (F)

ILM, CNRS UMR 5306, Univ Claude Bernard Lyon 1, Univ Lyon, 43 bd du 11 Nov 1918, 69622 Villeurbanne, France.

K Albespy (K)

Biom'up, 8, allée Irène Joliot Curie, 69800 Saint Priest, France.

V Centis (V)

Biom'up, 8, allée Irène Joliot Curie, 69800 Saint Priest, France.

D Campos (D)

Biom'up, 8, allée Irène Joliot Curie, 69800 Saint Priest, France.

S Roques (S)

CIC1401, CHU Bordeaux, Inserm, Univ Bordeaux, 146 rue Léo Saignat, 33000 Bordeaux, France.

M Meulle (M)

CIC1401, CHU Bordeaux, Inserm, Univ Bordeaux, 146 rue Léo Saignat, 33000 Bordeaux, France.

M Renard (M)

CIC1401, CHU Bordeaux, Inserm, Univ Bordeaux, 146 rue Léo Saignat, 33000 Bordeaux, France.

M Durand (M)

CIC1401, CHU Bordeaux, Inserm, Univ Bordeaux, 146 rue Léo Saignat, 33000 Bordeaux, France; BIOTIS Inserm U1026, Univ Bordeaux, Bioingénierie tissulaire, 146 rue Léo Saignat, Bordeaux 33000, France.

Q Denost (Q)

CIC1401, CHU Bordeaux, Inserm, Univ Bordeaux, 146 rue Léo Saignat, 33000 Bordeaux, France; BIOTIS Inserm U1026, Univ Bordeaux, Bioingénierie tissulaire, 146 rue Léo Saignat, Bordeaux 33000, France.

L Bordenave (L)

CIC1401, CHU Bordeaux, Inserm, Univ Bordeaux, 146 rue Léo Saignat, 33000 Bordeaux, France; BIOTIS Inserm U1026, Univ Bordeaux, Bioingénierie tissulaire, 146 rue Léo Saignat, Bordeaux 33000, France.

M Vandamme (M)

Voxcan, Marcy l'Etoile 69280, France.

E Chereul (E)

Voxcan, Marcy l'Etoile 69280, France.

M Vandesteene (M)

MDB Texinov, Saint-Didier-de-la Tour 38110, France.

N Boucard (N)

MDB Texinov, Saint-Didier-de-la Tour 38110, France.

L David (L)

IMP, CNRS UMR 5223, Univ Claude Bernard Lyon 1, Univ Lyon, 15 bd Latarjet, 69622 Villeurbanne, France.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Self-crosslinked fibrous collagen/chitosan...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Matériaux biocompatibles › Self-crosslinked fibrous collagen/chitosan...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Survie cellulaire › Self-crosslinked fibrous collagen/chitosan...
questionsmedicales.fr › Phénomènes chimiques › Self-crosslinked fibrous collagen/chitosan...
questionsmedicales.fr › Glucides › Polyosides › Chitine › Chitosane › Self-crosslinked fibrous collagen/chitosan...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Scléroprotéines › Protéines de la matrice extracellulaire › Collagène › Self-crosslinked fibrous collagen/chitosan...
questionsmedicales.fr › Techniques d'investigation › Test de matériaux › Self-crosslinked fibrous collagen/chitosan...
questionsmedicales.fr › Phénomènes physiques › Phénomènes mécaniques › Self-crosslinked fibrous collagen/chitosan...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Self-crosslinked fibrous collagen/chitosan...
questionsmedicales.fr › Techniques d'investigation › Imagerie optique › Self-crosslinked fibrous collagen/chitosan...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Spectrophotométrie › Spectrophotométrie IR › Spectroscopie infrarouge à transformée de Fourier › Self-crosslinked fibrous collagen/chitosan...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Thermogravimétrie › Self-crosslinked fibrous collagen/chitosan...
questionsmedicales.fr › Équipement et fournitures › Prothèses et implants › Structures d'échafaudage tissulaires › Self-crosslinked fibrous collagen/chitosan...