HAS2-AS1 is a novel LH/hCG target gene regulating HAS2 expression and enhancing cumulus cells migration.
Cell Movement
Cells, Cultured
Chorionic Gonadotropin
/ administration & dosage
Cumulus Cells
/ cytology
Female
Gene Expression
/ drug effects
Gene Expression Regulation
/ drug effects
Granulosa Cells
/ cytology
Humans
Hyaluronan Synthases
/ genetics
Ovary
/ metabolism
Ovulation
/ drug effects
RNA, Long Noncoding
/ genetics
RNA, Messenger
/ metabolism
RNA, Small Interfering
/ metabolism
Cumulus expansion
Cumulus migration
Granulosa cells
HAS2-AS1
Ovary
Journal
Journal of ovarian research
ISSN: 1757-2215
Titre abrégé: J Ovarian Res
Pays: England
ID NLM: 101474849
Informations de publication
Date de publication:
28 Feb 2019
28 Feb 2019
Historique:
received:
17
12
2018
accepted:
18
02
2019
entrez:
2
3
2019
pubmed:
2
3
2019
medline:
2
5
2019
Statut:
epublish
Résumé
The cumulus expansion process is one of the LH mediated ovulatory processes. Hyaluronan synthase 2 (HAS2) regulates the synthesis of hyaluronic acid, the main component of the cumulus expansion process. Recently, the lncRNA HAS2 antisense RNA 1 (HAS2-AS1) was identified in our global transcriptome RNA-sequencing of novel ovulation associated genes. The role of HAS2-AS1 in HAS2 regulation w.as studied previously with contradictive results in different models but not in the ovary. Taken together the induction of HAS2-AS1 and the important role of HAS2 in the cumulus expansion process, we hypothesize that HAS2-AS1 regulate HAS2 expression and function in the ovary. Therefore we undertook to study the expression, regulation, and possible functional role of HAS2-AS1 in the human ovary. HAS2-AS1, located within the HAS2 gene that was highly regulated in our library. We found that HAS2-AS1 express mainly in cumulus cells (CCs). Furthermore, HAS2-AS1 showed low expression in immature CCs and a significant increase expression in mature CCs. Functional studies reveal that inhibition of HAS2-AS1 by siRNA caused decrease expression of HAS2. Furthermore, inhibition of HAS2-AS1 by siRNA results in decrease migration of granulosa cells. Our results suggest that HAS2-AS1 is an LH/hCG target gene that plays a positive role in HAS2 expression and thus might play a role in regulating cumulus expansion and migration.
Sections du résumé
BACKGROUND
BACKGROUND
The cumulus expansion process is one of the LH mediated ovulatory processes. Hyaluronan synthase 2 (HAS2) regulates the synthesis of hyaluronic acid, the main component of the cumulus expansion process. Recently, the lncRNA HAS2 antisense RNA 1 (HAS2-AS1) was identified in our global transcriptome RNA-sequencing of novel ovulation associated genes. The role of HAS2-AS1 in HAS2 regulation w.as studied previously with contradictive results in different models but not in the ovary. Taken together the induction of HAS2-AS1 and the important role of HAS2 in the cumulus expansion process, we hypothesize that HAS2-AS1 regulate HAS2 expression and function in the ovary. Therefore we undertook to study the expression, regulation, and possible functional role of HAS2-AS1 in the human ovary.
RESULTS
RESULTS
HAS2-AS1, located within the HAS2 gene that was highly regulated in our library. We found that HAS2-AS1 express mainly in cumulus cells (CCs). Furthermore, HAS2-AS1 showed low expression in immature CCs and a significant increase expression in mature CCs. Functional studies reveal that inhibition of HAS2-AS1 by siRNA caused decrease expression of HAS2. Furthermore, inhibition of HAS2-AS1 by siRNA results in decrease migration of granulosa cells.
CONCLUSIONS
CONCLUSIONS
Our results suggest that HAS2-AS1 is an LH/hCG target gene that plays a positive role in HAS2 expression and thus might play a role in regulating cumulus expansion and migration.
Identifiants
pubmed: 30819231
doi: 10.1186/s13048-019-0495-3
pii: 10.1186/s13048-019-0495-3
pmc: PMC6396505
doi:
Substances chimiques
Chorionic Gonadotropin
0
RNA, Long Noncoding
0
RNA, Messenger
0
RNA, Small Interfering
0
long noncoding RNA HAS2-AS1, human
0
HAS2 protein, human
EC 2.4.1.212
Hyaluronan Synthases
EC 2.4.1.212
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
21Subventions
Organisme : Ministry of Health, Israel
ID : 3-00000-7410
Organisme : Chaim Sheba Medical Center
ID : 05/2014
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