Using Ubiquitin Binders to Decipher the Ubiquitin Code.

Animals Humans Protein Binding Protein Processing, Post-Translational Ubiquitin / metabolism Ubiquitination
TUBE affimers autophagy–lysosome system proteolysis signaling ubiquitin code ubiquitin-binding domain ubiquitin–proteasome system

Journal

Trends in biochemical sciences
ISSN: 0968-0004
Titre abrégé: Trends Biochem Sci
Pays: England
ID NLM: 7610674

Informations de publication

Date de publication:
07 2019
Historique:
received: 26 11 2018
revised: 23 01 2019
accepted: 29 01 2019
pubmed: 2 3 2019
medline: 18 9 2020
entrez: 2 3 2019
Statut: ppublish

Résumé

Post-translational modifications (PTMs) by ubiquitin (Ub) are versatile, highly dynamic, and involved in nearly all aspects of eukaryote biological function. The reversibility and heterogeneity of Ub chains attached to protein substrates have complicated their isolation, quantification, and characterization. Strategies have emerged to isolate endogenous ubiquitylated targets, including technologies based on the use of Ub-binding peptides, such as tandem-repeated Ub-binding entities (TUBEs). TUBEs allow the identification and characterization of Ub chains, and novel substrates for deubiquitylases (DUBs) and Ub ligases (E3s). Here we review their impact on purification, analysis of pan or chain-selective polyubiquitylated proteins and underline the biological relevance of this information. Together with peptide aptamers and other Ub affinity-based approaches, TUBEs will contribute to unraveling the secrets of the Ub code.

Identifiants

DOI: 10.1016/j.tibs.2019.01.011 PMID: 30819414
pubmed: 30819414
pii: S0968-0004(19)30020-9
doi: 10.1016/j.tibs.2019.01.011
pii:
doi:

Substances chimiques

Ubiquitin 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

599-615

Informations de copyright

Copyright © 2019 Elsevier Ltd. All rights reserved.

Auteurs

Michael Mattern (M)

Progenra Inc., 277 Great Valley Parkway, Malvern 19355, Pennsylvania, USA; These authors contributed equally.

James Sutherland (J)

CIC bioGUNE, Technology Park of Bizkaia, Bldg. 801A, 48160 Derio, Spain; These authors contributed equally.

Karteek Kadimisetty (K)

LifeSensors Inc., 271 Great Valley Parkway, Malvern 19355, Pennsylvania, USA.

Rosa Barrio (R)

CIC bioGUNE, Technology Park of Bizkaia, Bldg. 801A, 48160 Derio, Spain.

Manuel S Rodriguez (MS)

ITAV-IPBS-UPS CNRS USR3505, 1 place Pierre Potier, Oncopole entrée B, 31106 Toulouse, France. Electronic address: Manuel.RODRIGUEZ@itav.fr.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Using Ubiquitin Binders to Decipher the Ubiquitin Code.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Using Ubiquitin Binders to Decipher the Ubiquitin Code.
questionsmedicales.fr › Métabolisme › Liaison aux protéines › Using Ubiquitin Binders to Decipher the Ubiquitin Code.
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Modification traductionnelle des protéines › Maturation post-traductionnelle des protéines › Using Ubiquitin Binders to Decipher the Ubiquitin Code.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Ubiquitines › Ubiquitine › Using Ubiquitin Binders to Decipher the Ubiquitin Code.
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Modification traductionnelle des protéines › Maturation post-traductionnelle des protéines › Ubiquitination › Using Ubiquitin Binders to Decipher the Ubiquitin Code.