Non-muscle Myosin-II Is Required for the Generation of a Constriction Site for Subsequent Abscission.
Cell Biology
Functional Aspects of Cell Biology
Molecular Biology
Journal
iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038
Informations de publication
Date de publication:
29 Mar 2019
29 Mar 2019
Historique:
received:
06
03
2018
revised:
07
01
2019
accepted:
11
02
2019
pubmed:
3
3
2019
medline:
3
3
2019
entrez:
3
3
2019
Statut:
ppublish
Résumé
It remains unknown when, where, and how the site of abscission is generated during cytokinesis. Here, we show that the sites of constriction, i.e., the sites of future abscission, are initially formed at the ends of the intercellular bridge during early midbody stage, and that these sites are associated with the non-muscle myosin-IIB (not myosin-IIA), actin filaments, and septin 9 until abscission. The ESCRT-III component CHMP4B localizes to the midbody and "spreads" to the site of abscission only during late midbody stage. Strikingly, inhibition of myosin-II motor activity by a low dose of Blebbistatin completely abolishes the formation of the constriction sites, resulting in the localization of all the above-mentioned components to the midbody region. These data strongly suggest that a secondary actomyosin ring provides the primary driving force for the thinning of the intercellular bridge to allow ESCRT-mediated membrane fission.
Identifiants
pubmed: 30825839
pii: S2589-0042(19)30044-6
doi: 10.1016/j.isci.2019.02.010
pmc: PMC6396101
pii:
doi:
Types de publication
Journal Article
Langues
eng
Pagination
69-81Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM115420
Pays : United States
Informations de copyright
Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved.
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