Extracellular vesicles in synovial fluid from juvenile horses: No age-related changes in the quantitative profile.

Animals Animals, Newborn Collagen Type II / metabolism Glycosaminoglycans / metabolism Horses / growth & development Matrix Metalloproteinases / metabolism Synovial Fluid / metabolism
Biomarker CD44 Foal Joint development Joint homeostasis

Journal

Veterinary journal (London, England : 1997)
ISSN: 1532-2971
Titre abrégé: Vet J
Pays: England
ID NLM: 9706281

Informations de publication

Date de publication:
Feb 2019
Historique:
received: 13 12 2017
revised: 29 09 2018
accepted: 07 12 2018
entrez: 4 3 2019
pubmed: 4 3 2019
medline: 22 3 2019
Statut: ppublish

Résumé

Extracellular vesicle (EV) concentration, characteristics and function in equine synovial fluid (SF) during normal growth and development has not previously been studied. Isolation of EVs was performed in SF from three healthy foals and two adult horses by differential ultracentrifugation (10,000g and 200,000g); EVs were purified by sucrose density gradient floatation and analysed by high-resolution flow cytometry (FCM), buoyant density and western blotting. Additionally, repeated biomarker analysis of sulphated glycosaminoglycans (GAG), matrix metalloproteinase (MMP), C-terminal crosslinked telopeptide type II collagen (CTX-II), collagenase cleaved neopeptide type II collagen (C2C) was performed in SF from 10 foals and six adult horses. In contrast with the quantitative EV profile, the biomarker profile in SF from juvenile joints was substantially different from that in SF from adult animals. However, there were qualitative differences in the high-resolution FCM scatter plots. Future in-depth functional analyses may reveal differences between juvenile and mature EVs in SF.

Identifiants

DOI: 10.1016/j.tvjl.2018.12.010 PMID: 30825901 PMC: PMC7116028
pubmed: 30825901
pii: S1090-0233(18)30775-5
doi: 10.1016/j.tvjl.2018.12.010
pmc: PMC7116028
mid: EMS94320
pii:
doi:

Substances chimiques

Collagen Type II 0
Glycosaminoglycans 0
Matrix Metalloproteinases EC 3.4.24.-

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

91-93

Subventions

Organisme : European Research Council
ID : 647426
Pays : International

Informations de copyright

Copyright © 2018. Published by Elsevier Ltd.

Références

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pubmed: 23584393
J Extracell Vesicles. 2016 Aug 09;5:31751
pubmed: 27511891
Ann Rheum Dis. 1998 Nov;57(11):697-9
pubmed: 9924215
Nat Rev Rheumatol. 2016 Apr;12(4):243-9
pubmed: 26729461
Osteoarthritis Cartilage. 2016 Dec;24(12):2135-2140
pubmed: 27390028
Sci Rep. 2016 Oct 27;6:35928
pubmed: 27786276
Vet J. 2011 Dec;190(3):390-5
pubmed: 21216637
Nat Rev Rheumatol. 2014 Jun;10(6):356-64
pubmed: 24535546
Osteoarthritis Cartilage. 2010 Nov;18(11):1467-76
pubmed: 21056352
Am J Vet Res. 2004 Mar;65(3):296-302
pubmed: 15027675

Auteurs

J Boere (J)

Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 112, 3584CM Utrecht, The Netherlands.

C H A van de Lest (CHA)

Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 112, 3584CM Utrecht, The Netherlands; Department of Biochemistry & Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 2, 3584CM Utrecht, The Netherlands.

J C de Grauw (JC)

Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 112, 3584CM Utrecht, The Netherlands.

S G M Plomp (SGM)

Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 112, 3584CM Utrecht, The Netherlands.

S F W M Libregts (SFWM)

Department of Biochemistry & Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 2, 3584CM Utrecht, The Netherlands.

G J A Arkesteijn (GJA)

Department of Biochemistry & Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 2, 3584CM Utrecht, The Netherlands; Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584CM Utrecht, The Netherlands.

J Malda (J)

Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 112, 3584CM Utrecht, The Netherlands; Department of Orthopaedics, University Medical Center Utrecht, Utrecht University, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands.

M H M Wauben (MHM)

Department of Biochemistry & Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 2, 3584CM Utrecht, The Netherlands.

P R van Weeren (PR)

Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 112, 3584CM Utrecht, The Netherlands. Electronic address: r.vanweeren@uu.nl.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Extracellular vesicles in synovial fluid from...
questionsmedicales.fr › Eucaryotes › Animaux › Groupes animaux › Animaux nouveau-nés › Extracellular vesicles in synovial fluid from...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Scléroprotéines › Protéines de la matrice extracellulaire › Collagène › Collagènes fibrillaires › Collagène de type II › Extracellular vesicles in synovial fluid from...
questionsmedicales.fr › Glucides › Polyosides › Glycosaminoglycanes › Extracellular vesicles in synovial fluid from...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Perissodactyla › Equidae › Equus caballus › Extracellular vesicles in synovial fluid from...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Peptide hydrolases › Metalloproteases › Metalloendopeptidases › Matrix metalloproteinases › Extracellular vesicles in synovial fluid from...
questionsmedicales.fr › Liquides et sécrétions biologiques › Liquides biologiques › Liquide extracellulaire › Synovie › Extracellular vesicles in synovial fluid from...