Distinctive Krebs cycle remodeling in iPSC-derived neural and mesenchymal stem cells.
Differentiation
Induced pluripotent stem cells
Krebs cycle
Mitochondria
Journal
Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516
Informations de publication
Date de publication:
09 04 2019
09 04 2019
Historique:
received:
23
01
2019
accepted:
07
02
2019
pubmed:
4
3
2019
medline:
22
1
2020
entrez:
4
3
2019
Statut:
ppublish
Résumé
Mitochondria play a vital role in proliferation and differentiation and their remodeling in the course of differentiation is related to the variable energy and metabolic needs of the cell. In this work, we show a distinctive mitochondrial remodeling in human induced pluripotent stem cells differentiated into neural or mesenchymal progenitors. While leading to upregulation of the citrate synthase-α-ketoglutarate dehydrogenase segment of the Krebs cycle and increased respiratory chain activities and respiration in the mesenchymal stem cells, the remodeling in the neural stem cells resulted in downregulation of α-ketoglutarate dehydrogenase, upregulation of isocitrate dehydrogenase 2 and the accumulation of α-ketoglutarate. The distinct, lineage-specific changes indicate an involvement of these Krebs cycle enzymes in cell differentiation.
Identifiants
pubmed: 30826061
pii: S0006-291X(19)30220-7
doi: 10.1016/j.bbrc.2019.02.033
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
658-664Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.