Double-stranded sperm DNA damage is a cause of delay in embryo development and can impair implantation rates.
Adult
DNA
/ genetics
DNA Damage
/ physiology
Double-Blind Method
Embryo Implantation
/ genetics
Embryonic Development
/ genetics
Female
Fertilization in Vitro
Humans
Infertility
/ genetics
Male
Pregnancy
Pregnancy Rate
Prospective Studies
Sperm Injections, Intracytoplasmic
Spermatozoa
/ metabolism
Time-Lapse Imaging
DNA damage
Sperm
embryo kinetics
implantation
male factor
Journal
Fertility and sterility
ISSN: 1556-5653
Titre abrégé: Fertil Steril
Pays: United States
ID NLM: 0372772
Informations de publication
Date de publication:
04 2019
04 2019
Historique:
received:
28
03
2018
revised:
26
11
2018
accepted:
26
11
2018
pubmed:
4
3
2019
medline:
3
8
2019
entrez:
4
3
2019
Statut:
ppublish
Résumé
To analyze the effect of single- and double-stranded sperm DNA fragmentation (ssSDF and dsSDF) on human embryo kinetics monitored under a time-lapse system. Observational, double blind, prospective cohort study. University spin-off and private center. One hundred ninety-six embryos from 43 infertile couples were included prospectively. None. SsSDF and dsSDF were analyzed in the same semen sample used for intracytoplasmic sperm injection. Embryo kinetics was then monitored using time-lapse technology, and the timing of each embryo division was obtained. When comparing embryos obtained from semen samples with low dsSDF and high dsSDF, splitting data using a statistically significant delay in high dsSDF was observed in second polar body extrusion, T4, T8, morula, and starting blastocyst and embryo implantation rates were impaired. Embryo kinetics and implantation rates are not significantly affected when high values of ssSDF are present. Different patterns of delay in embryo kinetics were observed for these different types of DNA damage: dsSDF caused a delay along all stages of embryo development; however, its major effect was observed at the second polar body extrusion and morula stages, coinciding with embryo DNA damage checkpoint activation as described before; ssSDF had its major effect at the pronucleus stage, but embryo kinetics was then restored at all following stages. The results show that dsSDF could be the main type of DNA damage that affects embryo development in intracytoplasmic sperm injection cycles, probably due to motility-based sperm selection in this assisted reproduction procedure. Double-stranded sperm DNA damage caused a delay in embryo development and impaired implantation, while single-stranded DNA damage did not significantly affect embryo kinetics and implantation.
Identifiants
pubmed: 30826116
pii: S0015-0282(18)32236-2
doi: 10.1016/j.fertnstert.2018.11.035
pii:
doi:
Substances chimiques
DNA
9007-49-2
Types de publication
Journal Article
Observational Study
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
699-707.e1Commentaires et corrections
Type : CommentIn
Type : CommentIn
Informations de copyright
Copyright © 2018 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.