The anti MRSA biofilm activity of Thymus vulgaris essential oil in nanovesicles.
Animals
Anti-Bacterial Agents
/ chemistry
Biofilms
/ drug effects
Halorubrum
/ chemistry
Humans
Macrophages
/ drug effects
Methicillin-Resistant Staphylococcus aureus
/ drug effects
Mice
Microbial Sensitivity Tests
Nanostructures
/ chemistry
Oils, Volatile
/ chemistry
Phosphatidylcholines
/ chemistry
Polysorbates
/ chemistry
Staphylococcal Infections
/ microbiology
Thymus Plant
/ chemistry
Antibiofilm activity
Nanovesicles
Thymus vulgaris essential oil
Journal
Phytomedicine : international journal of phytotherapy and phytopharmacology
ISSN: 1618-095X
Titre abrégé: Phytomedicine
Pays: Germany
ID NLM: 9438794
Informations de publication
Date de publication:
Apr 2019
Apr 2019
Historique:
received:
06
11
2018
revised:
15
12
2018
accepted:
20
12
2018
pubmed:
4
3
2019
medline:
25
6
2019
entrez:
4
3
2019
Statut:
ppublish
Résumé
Thymus vulgaris essential oil (T) could be an alternative to classical antibiotics against bacterial biofilms, which show increased tolerance to antibiotics and host defence systems and contribute to the persistence of chronic bacterial infections. A nanovesicular formulation of T may chemically protect the structure and relative composition of its multiple components, potentially improving its antibacterial and antibiofilm activity. We prepared and structurally characterized T in two types of nanovesicles: nanoliposomes (L80-T) made of Soybean phosphatidylcholine (SPC) and Polysorbate 80 (P80) [SPC:P80:T 1:0.75:0.3 w:w], and nanoarchaeosomes (A80-T) made of SPC, P80 and total polar archaeolipids (TPA) extracted from archaebacteria Halorubrum tebenquichense [SPC:TPA:P80:T 0.5:0.50.75:0.7 w:w]. We determined the macrophage cytotoxicity and the antibacterial activity against Staphylococcus aureus ATCC 25,923 and four MRSA clinical strains. L80-T (Z potential -4.1 ± 0.6 mV, ∼ 115 nm, ∼ 22 mg/ml T) and A80-T (Z potential -6.6 ± 1.5 mV, ∼ 130 nm, ∼ 42 mg/ml T) were colloidally and chemically stable, maintaining size, PDI, Z potential and T concentration for at least 90 days. While MIC Overall, because of its lower MIC
Sections du résumé
BACKGROUND
BACKGROUND
Thymus vulgaris essential oil (T) could be an alternative to classical antibiotics against bacterial biofilms, which show increased tolerance to antibiotics and host defence systems and contribute to the persistence of chronic bacterial infections.
HYPOTHESIS
OBJECTIVE
A nanovesicular formulation of T may chemically protect the structure and relative composition of its multiple components, potentially improving its antibacterial and antibiofilm activity.
STUDY DESIGN
METHODS
We prepared and structurally characterized T in two types of nanovesicles: nanoliposomes (L80-T) made of Soybean phosphatidylcholine (SPC) and Polysorbate 80 (P80) [SPC:P80:T 1:0.75:0.3 w:w], and nanoarchaeosomes (A80-T) made of SPC, P80 and total polar archaeolipids (TPA) extracted from archaebacteria Halorubrum tebenquichense [SPC:TPA:P80:T 0.5:0.50.75:0.7 w:w]. We determined the macrophage cytotoxicity and the antibacterial activity against Staphylococcus aureus ATCC 25,923 and four MRSA clinical strains.
RESULTS
RESULTS
L80-T (Z potential -4.1 ± 0.6 mV, ∼ 115 nm, ∼ 22 mg/ml T) and A80-T (Z potential -6.6 ± 1.5 mV, ∼ 130 nm, ∼ 42 mg/ml T) were colloidally and chemically stable, maintaining size, PDI, Z potential and T concentration for at least 90 days. While MIC
CONCLUSION
CONCLUSIONS
Overall, because of its lower MIC
Identifiants
pubmed: 30826631
pii: S0944-7113(18)30619-6
doi: 10.1016/j.phymed.2018.12.025
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Oils, Volatile
0
Phosphatidylcholines
0
Polysorbates
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
339-351Informations de copyright
Copyright © 2019. Published by Elsevier GmbH.