Improved features of a highly stable protease from Penaeus vannamei by immobilization on glutaraldehyde activated graphene oxide nanosheets.
Enzyme immobilization
Enzyme stabilization
Glutaraldehyde
Graphene oxide nanosheets
Penaeus vannamei protease
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
01 Jun 2019
01 Jun 2019
Historique:
received:
23
01
2019
revised:
05
02
2019
accepted:
28
02
2019
pubmed:
5
3
2019
medline:
6
8
2019
entrez:
5
3
2019
Statut:
ppublish
Résumé
In this study, we report the synthesis of graphene oxide nanosheets (GON) by a modified Hummers method. Then, a protease purified from the Penaeus vannamei shrimp was immobilized on the GON activated with glutaraldehyde. Several techniques such as SEM, DLS and FTIR were applied to characterize the different nano-structures at the different levels. The immobilization of the protease on the GON activated with glutaraldehyde did not affect the optimum pH, but significantly improved thermal stability and stability at extreme pH values, as well as activity at 90 °C. After 24 h of incubation at 90 °C, the free enzyme retained less than 10% of the activity, while the immobilized enzyme kept more than 90% of its original activity. The apparent K
Identifiants
pubmed: 30831167
pii: S0141-8130(19)30561-6
doi: 10.1016/j.ijbiomac.2019.02.163
pii:
doi:
Substances chimiques
Enzymes, Immobilized
0
Oxides
0
Graphite
7782-42-5
Peptide Hydrolases
EC 3.4.-
Glutaral
T3C89M417N
Types de publication
Journal Article
Langues
eng
Pagination
564-572Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.