Cohesin-Mediated Genome Architecture Does Not Define DNA Replication Timing Domains.
Chromatin interactions 4
Cohesin 2
DNA
Genome architecture 3
Replication timing 1
Journal
Genes
ISSN: 2073-4425
Titre abrégé: Genes (Basel)
Pays: Switzerland
ID NLM: 101551097
Informations de publication
Date de publication:
04 03 2019
04 03 2019
Historique:
received:
14
01
2019
revised:
07
02
2019
accepted:
19
02
2019
entrez:
7
3
2019
pubmed:
7
3
2019
medline:
7
3
2019
Statut:
epublish
Résumé
3D genome organization is strongly predictive of DNA replication timing in mammalian cells. This work tested the extent to which loop-based genome architecture acts as a regulatory unit of replication timing by using an auxin-inducible system for acute cohesin ablation. Cohesin ablation in a population of cells in asynchronous culture was shown not to disrupt patterns of replication timing as assayed by replication sequencing (RepliSeq) or BrdU-focus microscopy. Furthermore, cohesin ablation prior to S phase entry in synchronized cells was similarly shown to not impact replication timing patterns. These results suggest that cohesin-mediated genome architecture is not required for the execution of replication timing patterns in S phase, nor for the establishment of replication timing domains in G1.
Identifiants
pubmed: 30836708
pii: genes10030196
doi: 10.3390/genes10030196
pmc: PMC6471042
pii:
doi:
Substances chimiques
Cell Cycle Proteins
0
DNA-Binding Proteins
0
Indoleacetic Acids
0
RAD21 protein, human
0
Bromodeoxyuridine
G34N38R2N1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/N016858/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 203727/Z/16/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 110064/Z/15/Z
Pays : United Kingdom
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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