TGF-
Chondrogenesis
Mesenchymal stem cells
Receptor
Terminal differentiation
Journal
International journal of stem cells
ISSN: 2005-3606
Titre abrégé: Int J Stem Cells
Pays: Korea (South)
ID NLM: 101497587
Informations de publication
Date de publication:
30 Mar 2019
30 Mar 2019
Historique:
received:
27
12
2018
revised:
21
12
2018
accepted:
28
12
2018
pubmed:
7
3
2019
medline:
7
3
2019
entrez:
7
3
2019
Statut:
ppublish
Résumé
Mesenchymal stem cells (MSCs) become hypertrophic in long term despite chondrogenic differentiation following the pathway of growth plate chondrocytes. This terminal differentiation leads to phenotypically unstable cartilage and was mirrored in vitro by addition of hypertrophy inducing medium. We investigated how intrinsic TGF- Human bone marrow derived MSC were chondrogenically differentiated in 3D culture. At day 14 medium conditions were changed to 1. pro-hypertrophic by addition of T3 and withdrawal of TGF- Our results suggest that the enhancement of hypertrophy in this model is a result of both activation of pro-hypertrophic BMP signaling and reduction of anti-hypertrophic TGF
Sections du résumé
BACKGROUND AND OBJECTIVES
OBJECTIVE
Mesenchymal stem cells (MSCs) become hypertrophic in long term despite chondrogenic differentiation following the pathway of growth plate chondrocytes. This terminal differentiation leads to phenotypically unstable cartilage and was mirrored in vitro by addition of hypertrophy inducing medium. We investigated how intrinsic TGF-
METHODS AND RESULTS
RESULTS
Human bone marrow derived MSC were chondrogenically differentiated in 3D culture. At day 14 medium conditions were changed to 1. pro-hypertrophic by addition of T3 and withdrawal of TGF-
CONCLUSIONS
CONCLUSIONS
Our results suggest that the enhancement of hypertrophy in this model is a result of both activation of pro-hypertrophic BMP signaling and reduction of anti-hypertrophic TGF
Identifiants
pubmed: 30836731
pii: ijsc18088
doi: 10.15283/ijsc18088
pmc: PMC6457698
doi:
Types de publication
Journal Article
Langues
eng
Pagination
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