Cocaine Directly Inhibits α6-Containing Nicotinic Acetylcholine Receptors in Human SH-EP1 Cells and Mouse VTA DA Neurons.

SH-EP1 cells VTA, dopamine neurons, patch clamp cocaine nicotinic acetylcholine receptor

Journal

Frontiers in pharmacology
ISSN: 1663-9812
Titre abrégé: Front Pharmacol
Pays: Switzerland
ID NLM: 101548923

Informations de publication

Date de publication:
2019
Historique:
received: 06 10 2018
accepted: 21 01 2019
entrez: 7 3 2019
pubmed: 7 3 2019
medline: 7 3 2019
Statut: epublish

Résumé

Alpha6-containing nicotinic acetylcholine receptors are primarily found in neurons of the midbrain dopaminergic (DA) system, suggesting these receptors are potentially involved in drug reward and dependence. Here, we report a novel effect that cocaine directly inhibits α6N/α3Cβ2β3-nAChR (α6*-nAChRs) function. Human α6*-nAChRs were heterologously expressed within cells of the SH-EP1 cell line for functional characterization. Mechanically dissociated DA neurons from mouse ventral tegmental area (VTA) were used as a model of presynaptic α6*-nAChR activation since this method preserves terminal boutons. Patch-clamp recordings in whole-cell configuration were used to measure α6*-nAChR function as well as evaluate the effects of cocaine. In SH-EP1 cells containing heterologously expressed human α6*-nAChRs, cocaine inhibits nicotine-induced inward currents in a concentration-dependent manner with an IC

Identifiants

pubmed: 30837868
doi: 10.3389/fphar.2019.00072
pmc: PMC6383119
doi:

Types de publication

Journal Article

Langues

eng

Pagination

72

Subventions

Organisme : NIDA NIH HHS
ID : R01 DA011064
Pays : United States
Organisme : NIDA NIH HHS
ID : R01 DA042749
Pays : United States

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Auteurs

Dejie Chen (D)

Department of Neurology, Yunfu People's Hospital, Yunfu, China.
Department of Neurology, Yunfu People's Hospital, Yunfu, China.

Fenfei Gao (F)

Department of Neurology, Yunfu People's Hospital, Yunfu, China.

Xiaokuang Ma (X)

Division of Neurobiology, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, Phoenix, AZ, United States.
Department of Pharmacology, Shantou University Medical College, Shantou, China.

Jason Brek Eaton (JB)

Division of Neurobiology, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, Phoenix, AZ, United States.

Yuanbing Huang (Y)

Department of Neurology, Yunfu People's Hospital, Yunfu, China.
Division of Neurobiology, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, Phoenix, AZ, United States.

Ming Gao (M)

Division of Neurobiology, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, Phoenix, AZ, United States.

Yongchang Chang (Y)

Division of Neurobiology, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, Phoenix, AZ, United States.

Zegang Ma (Z)

Department of Physiology, Qingdao University of Medical College, Qingdao, China.

Taleen Der-Ghazarian (T)

School of Life Sciences, Arizona State University, Tempe, AZ, United States.

Janet Neisewander (J)

School of Life Sciences, Arizona State University, Tempe, AZ, United States.

Paul Whiteaker (P)

Division of Neurobiology, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, Phoenix, AZ, United States.

Jie Wu (J)

Department of Neurology, Yunfu People's Hospital, Yunfu, China.
Division of Neurobiology, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, Phoenix, AZ, United States.
Department of Pharmacology, Shantou University Medical College, Shantou, China.

Quanxi Su (Q)

Department of Neurology, Yunfu People's Hospital, Yunfu, China.

Classifications MeSH