Structural and functional characterization of a novel acidophilic 7α-hydroxysteroid dehydrogenase.
7α-hydroxysteroid dehydrogenase
acidophilic
bile acids
short-chain dehydrogenases/reductases
Journal
Protein science : a publication of the Protein Society
ISSN: 1469-896X
Titre abrégé: Protein Sci
Pays: United States
ID NLM: 9211750
Informations de publication
Date de publication:
05 2019
05 2019
Historique:
received:
14
09
2018
revised:
21
02
2019
accepted:
22
02
2019
pubmed:
7
3
2019
medline:
31
3
2020
entrez:
7
3
2019
Statut:
ppublish
Résumé
7α-Hydroxysteroid dehydrogenase (7α-HSDH) is an NAD(P)H-dependent oxidoreductase belonging to the short-chain dehydrogenases/reductases. In vitro, 7α-HSDH is involved in the efficient biotransformation of taurochenodeoxycholic acid (TCDCA) to tauroursodeoxycholic acid (TUDCA). In this study, a gene encoding novel 7α-HSDH (named as St-2-1) from fecal samples of black bear was cloned and heterologously expressed in Escherichia coli. The protein has subunits of 28.3 kDa and a native size of 56.6 kDa, which suggested a homodimer. We studied the relevant properties of the enzyme, including the optimum pH, optimum temperature, thermal stability, activators, and inhibitors. Interestingly, the data showed that St-2-1 differs from the 7α-HSDHs reported in the literature, as it functions under acidic conditions. The enzyme displayed its optimal activity at pH 5.5 (TCDCA). The acidophilic nature of 7α-HSDH expands its application environment and the natural enzyme bank of HSDHs, providing a promising candidate enzyme for the biosynthesis of TUDCA or other related chemical entities.
Identifiants
pubmed: 30839141
doi: 10.1002/pro.3599
pmc: PMC6460000
doi:
Substances chimiques
Taurochenodeoxycholic Acid
516-35-8
ursodoxicoltaurine
60EUX8MN5X
Hydroxysteroid Dehydrogenases
EC 1.1.-
7 alpha-hydroxysteroid dehydrogenase
EC 1.1.1.159
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
910-919Informations de copyright
© 2019 The Protein Society.
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