Comparison of Two PCR Assays for Trichomonas vaginalis.

Humans Male Molecular Diagnostic Techniques / methods Polymerase Chain Reaction / methods Prostatic Diseases / diagnosis Sensitivity and Specificity Trichomonas Infections / diagnosis Trichomonas vaginalis / genetics Urine / parasitology
DNA isolation PCR Trichomonas vaginalis

Journal

The Korean journal of parasitology
ISSN: 1738-0006
Titre abrégé: Korean J Parasitol
Pays: Korea (South)
ID NLM: 9435800

Informations de publication

Date de publication:
Feb 2019
Historique:
received: 19 11 2018
accepted: 31 01 2019
entrez: 7 3 2019
pubmed: 7 3 2019
medline: 13 4 2019
Statut: ppublish

Résumé

PCR is known to be the most sensitive method for diagnosing Trichomonas vaginalis infections. This study aimed to compare the sensitivity of a PCR assay for trichomoniasis (HY-PCR) developed in Hanyang University with the use of a Seeplex Ace Detection Kit®, using urine collected from four Korean men with prostatic disease. Overall, HY-PCR was more sensitive than the Seeplex Kit. The use of Chelex 100 is recommended for DNA isolation in order to increase the sensitivity of the PCR test.

Identifiants

DOI: 10.3347/kjp.2019.57.1.27 PMID: 30840796 PMC: PMC6409213
pubmed: 30840796
pii: kjp.2019.57.1.27
doi: 10.3347/kjp.2019.57.1.27
pmc: PMC6409213
doi:

Types de publication

Comparative Study Evaluation Study Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

27-31

Subventions

Organisme : National Research Foundation of Korea
ID : 2017R1A2B4002072
Organisme : Ministry of Science and ICT

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Auteurs

Chang-Suk Noh (CS)

Department of Internal Medicine, Hallym University Hangang Sacred Heart Hospital, Seoul 07247, Korea.

Sang-Su Kim (SS)

Department of Environmental Biology and Medical Parasitology, Hanyang University College of Medicine, Seoul 04763, Korea.
Department of Biomedical Science, Hanyang University Graduate School of Biomedical Science and Engineering, Hanyang University College of Medicine, Seoul 04763, Korea.

Sung-Yul Park (SY)

Department of Urology, Hanyang University College of Medicine, Seoul 04763, Korea.

Hong-Sang Moon (HS)

Department of Urology, Hanyang University College of Medicine, Seoul 04763, Korea.

Yeonchul Hong (Y)

Department of Parasitology and Tropical Medicine, Kyungpook National University School of Medicine, Daegu 41944, Korea.

Jae-Sook Ryu (JS)

Department of Environmental Biology and Medical Parasitology, Hanyang University College of Medicine, Seoul 04763, Korea.
Department of Biomedical Science, Hanyang University Graduate School of Biomedical Science and Engineering, Hanyang University College of Medicine, Seoul 04763, Korea.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Comparison of Two PCR Assays for Trichomonas vaginalis.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques de diagnostic moléculaire › Comparison of Two PCR Assays for Trichomonas vaginalis.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Comparison of Two PCR Assays for Trichomonas vaginalis.
questionsmedicales.fr › Maladies urogénitales › Maladies urogénitales de l'homme › Maladies de l'appareil génital mâle › Maladies de la prostate › Comparison of Two PCR Assays for Trichomonas vaginalis.
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Statistiques comme sujet › Sensibilité et spécificité › Comparison of Two PCR Assays for Trichomonas vaginalis.
questionsmedicales.fr › Infection › Maladies parasitaires › Protozooses › Trichomonase › Comparison of Two PCR Assays for Trichomonas vaginalis.
questionsmedicales.fr › Eucaryotes › Parabasalidea › Trichomonadida › Trichomonas › Trichomonas vaginalis › Comparison of Two PCR Assays for Trichomonas vaginalis.
questionsmedicales.fr › Liquides et sécrétions biologiques › Liquides biologiques › Urine › Comparison of Two PCR Assays for Trichomonas vaginalis.