MC159 of Molluscum Contagiosum Virus Suppresses Autophagy by Recruiting Cellular SH3BP4 via an SH3 Domain-Mediated Interaction.
Adaptor Proteins, Signal Transducing
/ metabolism
Apoptosis
/ drug effects
Autophagy
/ physiology
HEK293 Cells
HeLa Cells
Host-Pathogen Interactions
/ drug effects
Humans
Immune Evasion
/ drug effects
MCF-7 Cells
Molluscum Contagiosum
/ virology
Molluscum contagiosum virus
/ metabolism
NF-kappa B
/ metabolism
Protein Binding
Protein Processing, Post-Translational
/ drug effects
Signal Transduction
Viral Proteins
/ metabolism
src Homology Domains
/ physiology
MC159
MVC
SH3 domain
SH3BP4
autophagy
protein-protein interactions
vFLIP
Journal
Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724
Informations de publication
Date de publication:
15 05 2019
15 05 2019
Historique:
received:
12
09
2018
accepted:
20
02
2019
pubmed:
8
3
2019
medline:
3
3
2020
entrez:
8
3
2019
Statut:
epublish
Résumé
MC159 is a viral FLIP (FLICE inhibitory protein) encoded by the molluscum contagiosum virus (MCV) enabling MCV to evade antiviral immunity and to establish persistent infections in humans. Here, we show that MC159 contains a functional SH3 binding motif, which mediates avid and selective binding to SH3BP4, a signaling protein known to regulate endocytic trafficking and suppress cellular autophagy. The capacity to bind SH3BP4 was dispensable for regulation of NF-κB-mediated transcription and suppression of proapoptotic caspase activation but contributed to inhibition of amino acid starvation-induced autophagy by MC159. These results provide new insights into the cellular functions of MC159 and reveal SH3BP4 as a novel host cell factor targeted by a viral immune evasion protein.
Identifiants
pubmed: 30842330
pii: JVI.01613-18
doi: 10.1128/JVI.01613-18
pmc: PMC6498068
pii:
doi:
Substances chimiques
Adaptor Proteins, Signal Transducing
0
NF-kappa B
0
SH3BP4 protein, human
0
Viral Proteins
0
viral FLIP protein, Molluscum contagiosum virus
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2019 American Society for Microbiology.
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