Coexpression of CD163 and CD141 identifies human circulating IL-10-producing dendritic cells (DC-10).
Dendritic cells
IL-10
T regulatory type 1 (Tr1) cells
Tolerance
Journal
Cellular & molecular immunology
ISSN: 2042-0226
Titre abrégé: Cell Mol Immunol
Pays: China
ID NLM: 101242872
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
received:
30
08
2018
accepted:
08
02
2019
pubmed:
8
3
2019
medline:
3
6
2021
entrez:
8
3
2019
Statut:
ppublish
Résumé
Tolerogenic dendritic cells (DCs) are key players in maintaining immunological homeostasis, dampening immune responses, and promoting tolerance. DC-10, a tolerogenic population of human IL-10-producing DCs characterized by the expression of HLA-G and ILT4, play a pivotal role in promoting tolerance via T regulatory type 1 (Tr1) cells. Thus far, the absence of markers that uniquely identify DC-10 has limited in vivo studies. By in vitro gene expression profiling of differentiated human DCs, we identified CD141 and CD163 as surface markers for DC-10. The coexpression of CD141 and CD163 in combination with CD14 and CD16 enables the ex vivo isolation of DC-10 from the peripheral blood. CD14
Identifiants
pubmed: 30842629
doi: 10.1038/s41423-019-0218-0
pii: 10.1038/s41423-019-0218-0
pmc: PMC6952411
doi:
Substances chimiques
Antigens, CD
0
Antigens, Differentiation, Myelomonocytic
0
CD163 antigen
0
IL10 protein, human
0
Receptors, Cell Surface
0
THBD protein, human
0
Thrombomodulin
0
Interleukin-10
130068-27-8
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
95-107Références
Ziegler-Heitbrock, L. et al. Nomenclature of monocytes and dendritic cells in blood. Blood 116, e74–e80 (2010).
doi: 10.1182/blood-2010-02-258558
Mittag, D. et al. Human dendritic cell subsets from spleen and blood are similar in phenotype and function but modified by donor health status. J. Immunol. 186, 6207–6217 (2011).
doi: 10.4049/jimmunol.1002632
Jongbloed, S. L. et al. Human CD141 + (BDCA-3) + dendritic cells (DCs) represent a unique myeloid DC subset that cross-presents necrotic cell antigens. J. Exp. Med. 207, 1247–1260 (2010).
doi: 10.1084/jem.20092140
Siegal, F. P. et al. The nature of the principal type 1 interferon – producing cells in human blood. Science 284, 1835–1838 (1999).
doi: 10.1126/science.284.5421.1835
Moseman, E. A. et al. Human plasmacytoid dendritic cells activated by CpG oligodeoxynucleotides induce the generation of CD4 + CD25 + regulatory T cells. J. Immunol. 173, 4433–4442 (2004).
doi: 10.4049/jimmunol.173.7.4433
Ito, T. et al. Plasmacytoid dendritic cells prime IL-10-producing T regulatory cells by inducible costimulator ligand. J. Exp. Med. 204, 105–115 (2007).
doi: 10.1084/jem.20061660
Banchereau, J. & Steinman, R. M. Dendritic cells and the control of immunity. Nature 392, 245–252 (1998).
doi: 10.1038/32588
Iberg, C. A., Jones, A. & Hawiger, D. Dendritic cells as inducers of peripheral tolerance. Trends Immunol. 38, 793–804 (2017).
doi: 10.1016/j.it.2017.07.007
Gordon, J. R., Ma, Y., Churchman, L., Gordon, S. A. & Dawicki, W. Regulatory dendritic cells for immunotherapy in immunologic diseases. Front. Immunol. 5, 7 (2014).
doi: 10.3389/fimmu.2014.00007
Horton, C., Shanmugarajah, K. & Fairchild, P. J. Harnessing the properties of dendritic cells in the pursuit of immunological tolerance. Biomed. J. 40, 80–93 (2017).
doi: 10.1016/j.bj.2017.01.002
Waisman A., Lukas D., Clausen B. E., Yogev N. Dendritic cells as gatekeepers of tolerance. Semin Immunopathol 39, 1–11 (2017).
doi: 10.1007/s00281-016-0583-z
Gregori, S. et al. Differentiation of type 1 T regulatory cells (Tr1) by tolerogenic DC-10 requires the IL-10-dependent ILT4/HLA-G pathway. Blood 116, 935–944 (2010).
doi: 10.1182/blood-2009-07-234872
Amodio, G. et al. HLA-G expression levels influence the tolerogenic activity of human DC-10. Haematologica 100, 548–557 (2015).
doi: 10.3324/haematol.2014.113803
Bacchetta, R. et al. Molecular and functional characterization of allogantigen-specific anergic T cells suitable for cell therapy. Haematologica 95, 2134–2143 (2010).
doi: 10.3324/haematol.2010.025825
Petrelli, A. et al. Generation of donor-specific t regulatory type 1 cells from patients on dialysis for cell therapy after kidney transplantation. Transplantation 99, 1582–1589 (2015).
doi: 10.1097/TP.0000000000000751
Comi, M., Amodio, G. & Gregori, S. Interleukin-10-producing DC-10 Is a unique tool to promote tolerance via antigen-specific T regulatory type 1 cells. Front. Immunol. 9, 682 (2018).
doi: 10.3389/fimmu.2018.00682
Amodio, G. et al. HLA-G expressing DC-10 and CD4(+) T cells accumulate in human decidua during pregnancy. Hum. Immunol. 74, 406–411 (2013).
doi: 10.1016/j.humimm.2012.11.031
Repnik, U., Knezevic, M. & Jeras, M. Simple and cost-effective isolation of monocytes from buffy coats. J. Immunol. Methods 278, 283–292 (2003).
doi: 10.1016/S0022-1759(03)00231-X
Gagliani, N. et al. Coexpression of CD49b and LAG-3 identifies human and mouse T regulatory type 1 cells. Nat. Med. 19, 739–746 (2013).
doi: 10.1038/nm.3179
Gautier, L., Cope, L., Bolstad, B. M. & Irizarry, R. A. Affy - analysis of Affymetrix GeneChip data at the probe level. Bioinformatics 20, 307–315 (2004).
doi: 10.1093/bioinformatics/btg405
Ritchie, M. E. et al. Limma powers differential expression analyses for RNA-sequencing and microarray studies. Nucleic Acids Res. 43, e47 (2015).
doi: 10.1093/nar/gkv007
Jiang H., Lei R., Ding S. W., Zhu S. Skewer: A fast and accurate adapter trimmer for next-generation sequencing paired-end reads. BMC Bioinform. 2014; 15. https://doi.org/10.1186/1471-2105-15-182 .
Dobin, A. et al. STAR: Ultrafast universal RNA-seq aligner. Bioinformatics 29, 15–21 (2013).
doi: 10.1093/bioinformatics/bts635
Love M. I., Huber W., Anders S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol. 2014; 15. https://doi.org/10.1186/s13059-014-0550-8 .
Subramanian, A. et al. Gene set enrichment analysis: a knowledge-based approach for interpreting genome- wide expression profiles gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles. Proc. Natl Acad. Sci. USA 102, 15545–15550 (2005).
doi: 10.1073/pnas.0506580102
Chen E. Y., et al. Enrichr: interactive and collaborative HTML5 gene list enrichment analysis tool. BMC Bioinform. 2013, 14. https://doi.org/10.1186/1471-2105-14-128 .
doi: 10.1186/1471-2105-14-128
Supek F., Bošnjak M., Škunca N., Šmuc T. Revigo summarizes and visualizes long lists of gene ontology terms. PLoS One 2011, 6. https://doi.org/10.1371/journal.pone.0021800 .
doi: 10.1371/journal.pone.0021800
Mahnke, K., Schmitt, E., Bonifaz, L., Enk, A. H. & Jonuleit, H. Immature, but not inactive: the tolerogenic function of immature dendritic cells. Immunol. Cell Biol. 80, 477–483 (2002).
doi: 10.1046/j.1440-1711.2002.01115.x
Levings, M. K. et al. Differentiation of Tr1 cells by immature dendritic cells requires IL-10 but not CD25 + CD4 + Tr cells. Blood 105, 1162–1169 (2005).
doi: 10.1182/blood-2004-03-1211
Kumar, N. A. et al. The role of antigen presenting cells in the induction of HIV-1 latency in resting CD4(+) T-cells. Retrovirology 12, 76 (2015).
doi: 10.1186/s12977-015-0204-2
Macdonald, K. Pa et al. Characterization of human blood dendritic cell subsets. Blood 100, 4512–4520 (2002).
doi: 10.1182/blood-2001-11-0097
Gupta, M. R., Kolli, D. & Garofalo, R. P. Differential response of BDCA-1+ and BDCA-3+ myeloid dendritic cells to respiratory syncytial virus infection. Respir. Res. 14, 1–14 (2013).
doi: 10.1186/1465-9921-14-71
Chu, C.-C. et al. Resident CD141 (BDCA3)+ dendritic cells in human skin produce IL-10 and induce regulatory T cells that suppress skin inflammation. J. Exp. Med. 209, 935–945 (2012).
doi: 10.1084/jem.20112583
Williams, L., Jarai, G., Smith, A. & Finan, P. IL-10 expression profiling in human monocytes. J. Leukoc. Biol. 72, 800–809 (2002).
pubmed: 12377950
Philippidis, P. et al. Hemoglobin scavenger receptor CD163 mediates interleukin-10 release and heme oxygenase-1 synthesis: antiinflammatory monocyte-macrophage responses in vitro, in resolving skin blisters in vivo, and after cardiopulmonary bypass surgery. Circ. Res. 94, 119–126 (2004).
doi: 10.1161/01.RES.0000109414.78907.F9
Mosser, D. M. The many faces of macrophage activation. J. Leukoc. Biol. 73, 209–212 (2003).
doi: 10.1189/jlb.0602325
Maniecki, M. B., Møller, H. J., Moestrup, S. K. & Møller, B. K. CD163 positive subsets of blood dendritic cells: The scavenging macrophage receptors CD163 and CD91 are coexpressed on human dendritic cells and monocytes. Immunobiology 211, 407–417 (2006).
doi: 10.1016/j.imbio.2006.05.019
Barman, S. et al. Identification of a human intestinal myeloid cell subset that regulates gut homeostasis. Int. Immunol. 28, 533–545 (2016).
doi: 10.1093/intimm/dxw034
Balan, S. et al. Human XCR1
doi: 10.4049/jimmunol.1401243
Alcántara-Hernández, M. et al. High-dimensional phenotypic mapping of human dendritic cells reveals interindividual variation and tissue specialization. Immunity 47, 1037–1050.e6 (2017).
doi: 10.1016/j.immuni.2017.11.001
Meredith, M. M. et al. Expression of the zinc finger transcription factor zDC (Zbtb46, Btbd4) defines the classical dendritic cell lineage. J. Exp. Med. 209, 1153–1165 (2012).
doi: 10.1084/jem.20112675