Survival of human cadaver skin on severe combined immune deficiency pigs: Proof of concept.
Journal
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
ISSN: 1524-475X
Titre abrégé: Wound Repair Regen
Pays: United States
ID NLM: 9310939
Informations de publication
Date de publication:
07 2019
07 2019
Historique:
received:
27
09
2018
revised:
30
01
2019
accepted:
01
03
2019
pubmed:
8
3
2019
medline:
25
2
2020
entrez:
8
3
2019
Statut:
ppublish
Résumé
Transplantation of human xenografts onto immunocompromised mice is a powerful research tool for studying wound healing. However, differences in healing between humans and mice and their small size limit this model. We determined whether human cadaver skin xenografts transplanted onto pigs with severe combined immune deficiency (SCID) would survive and not be rejected. Meshed (1:1.5), cryopreserved human cadaver skin was transplanted onto 10 partial thickness dermatome wounds in each of two normal domestic pigs and two SCID pigs. Autografts (n = 2/animal) from the four animals were used as controls. In normal pigs, all autografts were engrafted and healed with a minimal, if any, inflammation and scarring. All human xenografts were rejected by the normal pigs within 5-11 days and associated with an intense T-cell inflammatory response. In contrast, both autografts and xenografts were engrafted and survived the 28-day study in the SCID pigs with a minimal inflammation and no gross scarring.
Identifiants
pubmed: 30843296
doi: 10.1111/wrr.12715
pmc: PMC6602863
mid: NIHMS1017883
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
426-430Subventions
Organisme : NIH HHS
ID : R24 OD019813
Pays : United States
Informations de copyright
© 2019 by the Wound Healing Society.
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