Long chain lipid hydroperoxides increase the glutathione redox potential through glutathione peroxidase 4.
Glutathione
Glutathione peroxidase 4
Hydroperoxide
Lipid peroxidation
Journal
Biochimica et biophysica acta. General subjects
ISSN: 1872-8006
Titre abrégé: Biochim Biophys Acta Gen Subj
Pays: Netherlands
ID NLM: 101731726
Informations de publication
Date de publication:
05 2019
05 2019
Historique:
received:
04
09
2018
revised:
08
02
2019
accepted:
03
03
2019
pubmed:
8
3
2019
medline:
4
12
2019
entrez:
8
3
2019
Statut:
ppublish
Résumé
Peroxidation of PUFAs by a variety of endogenous and xenobiotic electrophiles is a recognized pathophysiological process that can lead to adverse health effects. Although secondary products generated from peroxidized PUFAs have been relatively well studied, the role of primary lipid hydroperoxides in mediating early intracellular oxidative events is not well understood. Live cell imaging was used to monitor changes in glutathione (GSH) oxidation in HAEC expressing the fluorogenic sensor roGFP during exposure to 9-hydroperoxy-10E,12Z-octadecadienoic acid (9-HpODE), a biologically important long chain lipid hydroperoxide, and its secondary product 9-hydroxy-10E,12Z-octadecadienoic acid (9-HODE). The role of hydrogen peroxide (H Exposure to 9-HpODE caused a dose-dependent increase in GSH oxidation in HAEC that was independent of intracellular or extracellular H Long chain lipid hydroperoxides can directly alter cytosolic E These results reveal a previously unrecognized consequence of lipid peroxidation, which may provide insight into disease states involving lipid peroxidation in their pathogenesis.
Sections du résumé
BACKGROUND
Peroxidation of PUFAs by a variety of endogenous and xenobiotic electrophiles is a recognized pathophysiological process that can lead to adverse health effects. Although secondary products generated from peroxidized PUFAs have been relatively well studied, the role of primary lipid hydroperoxides in mediating early intracellular oxidative events is not well understood.
METHODS
Live cell imaging was used to monitor changes in glutathione (GSH) oxidation in HAEC expressing the fluorogenic sensor roGFP during exposure to 9-hydroperoxy-10E,12Z-octadecadienoic acid (9-HpODE), a biologically important long chain lipid hydroperoxide, and its secondary product 9-hydroxy-10E,12Z-octadecadienoic acid (9-HODE). The role of hydrogen peroxide (H
RESULTS
Exposure to 9-HpODE caused a dose-dependent increase in GSH oxidation in HAEC that was independent of intracellular or extracellular H
CONCLUSIONS
Long chain lipid hydroperoxides can directly alter cytosolic E
SIGNIFICANCE
These results reveal a previously unrecognized consequence of lipid peroxidation, which may provide insight into disease states involving lipid peroxidation in their pathogenesis.
Identifiants
pubmed: 30844486
pii: S0304-4165(19)30052-2
doi: 10.1016/j.bbagen.2019.03.002
pmc: PMC6823641
mid: NIHMS1526290
pii:
doi:
Substances chimiques
Linoleic Acids
0
Linoleic Acids, Conjugated
0
9-hydroxy-10,12-octadecadienoic acid
15514-85-9
9-hydroperoxy-11,12-octadecadienoic acid
63121-49-3
Hydrogen Peroxide
BBX060AN9V
Phospholipid Hydroperoxide Glutathione Peroxidase
EC 1.11.1.12
Glutathione Peroxidase
EC 1.11.1.9
Glutathione
GAN16C9B8O
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
950-959Subventions
Organisme : Intramural EPA
ID : EPA999999
Pays : United States
Organisme : NIEHS NIH HHS
ID : F31 ES029020
Pays : United States
Organisme : NIEHS NIH HHS
ID : P30 ES010126
Pays : United States
Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.
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