Temporal-controlled bioactive molecules releasing core-shell nano-system for tissue engineering strategies in endodontics.
Alkaline Phosphatase
/ metabolism
Animals
Biocompatible Materials
/ pharmacology
Cell Adhesion
/ drug effects
Cell Differentiation
/ drug effects
Cell Line
Cell Proliferation
/ drug effects
Cell Survival
/ drug effects
Chemotaxis
/ drug effects
Dental Papilla
/ cytology
Dexamethasone
/ pharmacology
Endodontics
/ methods
Guinea Pigs
Humans
Male
Nanoparticles
/ chemistry
Odontogenesis
/ drug effects
Stem Cells
/ cytology
Time Factors
Tissue Engineering
/ methods
Transforming Growth Factor beta1
/ pharmacology
Core-shell nano-system
Dexamethasone
Odontogenic differentiation
Stem cells from apical papilla
TGF-β1
Temporal-controlled release
Journal
Nanomedicine : nanotechnology, biology, and medicine
ISSN: 1549-9642
Titre abrégé: Nanomedicine
Pays: United States
ID NLM: 101233142
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
11
12
2018
revised:
09
02
2019
accepted:
13
02
2019
pubmed:
8
3
2019
medline:
4
12
2019
entrez:
8
3
2019
Statut:
ppublish
Résumé
Temporal-controlled release of bioactive molecules is of key importance in the clinical translation of tissue engineering techniques. We engineered a core-shell nano-system (TD-NS) that sequentially released transforming growth factor-β1 (TGF-β1), a chemotactic/proliferating growth factor and dexamethasone (Dex), an osteo/odontogenic agent in a temporal-controlled manner. In stage-1, there was a rapid release of TGF-β1, reaching a concentration of 2 ng/mL of TGF-β1 in 7 days to 14 days, which tapers subsequently. In stage-2, Dex was released linearly from 9 days to 28 days. The TD-NS group showed a significantly higher (P < 0.05) osteo/odontogenic differentiation compared to the control and free TGF-β1 group (Free-TD) that was further corroborated with animal models/histochemical examination. The findings from this study highlighted the potential of temporal-controlled delivery of TGF-β1 and Dex from a single nano-carrier to direct spatial and temporal-control for a cell-free tissue engineering strategy in the treatment of apical periodontitis.
Identifiants
pubmed: 30844574
pii: S1549-9634(19)30044-9
doi: 10.1016/j.nano.2019.02.013
pii:
doi:
Substances chimiques
Biocompatible Materials
0
Transforming Growth Factor beta1
0
Dexamethasone
7S5I7G3JQL
Alkaline Phosphatase
EC 3.1.3.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
11-20Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.