Device Fabrication and Fluorescent Labeling of Preterm Birth Biomarkers for Microchip Electrophoresis.
Cyclic olefin copolymer
Hot embossing
Laser induced fluorescence
Microfluidics
Photolithography
Point-of-care diagnostics
Rapid analysis
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
9
3
2019
pubmed:
9
3
2019
medline:
8
8
2019
Statut:
ppublish
Résumé
An unmet need exists for a clinical diagnostic to determine preterm birth (PTB) risk. Such an assessment is possible with high sensitivity and specificity using a panel of nine biomarkers. An integrated microfluidic analysis system for these biomarkers is being developed which includes microchip electrophoresis (μCE) separation. A t-shaped microchip device can be used to test the μCE portion of this integrated system to find appropriate separation conditions. These t-shaped microchips can be fabricated using photolithographically patterned Si templates and hot embossing. PTB biomarkers can be fluorescently labeled using an amine-reactive dye prior to μCE. The μCE conditions established using this t-shaped device should be useful in developing a complete integrated microfluidic system for PTB risk assessment.
Identifiants
pubmed: 30847791
doi: 10.1007/978-1-4939-9213-3_12
doi:
Substances chimiques
Biomarkers
0
Fluorescent Dyes
0
Silicon
Z4152N8IUI
Types de publication
Journal Article
Langues
eng
Pagination
175-184Références
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