Antimicrobial activity of leaf extracts and isolated constituents of Croton linearis.
Alkaloids
/ isolation & purification
Animals
Antiprotozoal Agents
/ isolation & purification
Caribbean Region
Cell Line
Chloroform
/ chemistry
Chromatography, High Pressure Liquid
Croton
/ chemistry
Humans
Inhibitory Concentration 50
Leishmania infantum
/ drug effects
Macrophages
Mass Spectrometry
Medicine, Traditional
/ methods
Parasitic Sensitivity Tests
Plant Extracts
/ isolation & purification
Plant Leaves
/ chemistry
Protozoan Infections
/ drug therapy
Trypanosoma cruzi
/ drug effects
Antimicrobial
Bioassay-guided isolation
Croton linearis
Cytotoxicity
Isoquinoline alkaloids
Journal
Journal of ethnopharmacology
ISSN: 1872-7573
Titre abrégé: J Ethnopharmacol
Pays: Ireland
ID NLM: 7903310
Informations de publication
Date de publication:
23 May 2019
23 May 2019
Historique:
received:
12
12
2018
revised:
15
01
2019
accepted:
21
01
2019
pubmed:
9
3
2019
medline:
20
8
2019
entrez:
9
3
2019
Statut:
ppublish
Résumé
Leaves of Croton linearis, known as "rosemary", are widely used in folk medicine in Caribbean countries to treat fever and colds (associated to infections). To evaluate the in vitro antimicrobial activity of extracts and fractions derived from C. linearis leaves. Bioassay-guided fractionation and isolation of compounds from an ethanolic extract of C. linearis, using flash chromatography and semi-preparative HPLC-DAD-MS (High Performance Liquid Chromatography - Diode Array Detection - Mass Spectrometry). Isolated compounds were characterized by MS, 1D- and 2D-NMR (Nuclear Magnetic Resonance) spectroscopy. The microdilution method with resazurin, as well as direct counting with an optical microscope, were used to determine the in vitro antimicrobial activity against bacteria, fungi and parasites. Moreover, the cytotoxicity on human fetal lung fibroblasts (MRC-5) was evaluated. The total extract and chloroform fraction (ClF) showed high activity against protozoa with IC The results show the antiprotozoal potential of the extract and some isolated constituents, which supports the use of this species in Caribbean folk medicine.
Identifiants
pubmed: 30849504
pii: S0378-8741(18)34766-4
doi: 10.1016/j.jep.2019.01.049
pii:
doi:
Substances chimiques
Alkaloids
0
Antiprotozoal Agents
0
Plant Extracts
0
Chloroform
7V31YC746X
Types de publication
Journal Article
Langues
eng
Pagination
250-257Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.