Optimized Histological Preparation of Ovary for Ovariole Counting in Africanized Honey Bee Queens (Hymenoptera: Apidae).
histotechnique
honey bee breeding
insect ovary
protocol time
Journal
Journal of insect science (Online)
ISSN: 1536-2442
Titre abrégé: J Insect Sci
Pays: United States
ID NLM: 101096396
Informations de publication
Date de publication:
01 Mar 2019
01 Mar 2019
Historique:
received:
22
09
2018
entrez:
10
3
2019
pubmed:
10
3
2019
medline:
15
3
2019
Statut:
ppublish
Résumé
Techniques for counting ovariole number in virgin and mated Apis mellifera L. queens have been described in previous studies. Having a systematic and fast way to collect this measurement can help accelerate bee breeding programs, because selection decisions can be taken faster. The aim of this work was to develop an efficient histological method to preserve ovaries that allows assessing the number of ovarioles in newly emerged virgin queens, and also in mated queens, in a shorter time than the methods already published. The proposed method resulted in images suitable for ovariole counting in both newly emerged and mated queens, and the total histological process took less than 10 h. This method provides the optimization of the histological procedure for research breeding programs that use ovariole number as selection criteria for improving reproduction and production traits.
Identifiants
pubmed: 30851034
pii: 5372555
doi: 10.1093/jisesa/iez013
pmc: PMC6408696
pii:
doi:
Types de publication
Evaluation Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© The Author(s) 2019. Published by Oxford University Press on behalf of Entomological Society of America.
Références
Braz J Biol. 2002 Nov;62(4A):641-9
pubmed: 12659014
J Insect Sci. 2011;11:82
pubmed: 21870968