Plugged into the Ku-DNA hub: The NHEJ network.

Conserved binding motifs DNA damage DNA repair machineries Double-strand breaks Ku NHEJ Protein interactions network

Journal

Progress in biophysics and molecular biology
ISSN: 1873-1732
Titre abrégé: Prog Biophys Mol Biol
Pays: England
ID NLM: 0401233

Informations de publication

Date de publication:
10 2019
Historique:
received: 31 10 2018
revised: 26 02 2019
accepted: 01 03 2019
pubmed: 10 3 2019
medline: 19 5 2020
entrez: 10 3 2019
Statut: ppublish

Résumé

In vertebrates, double-strand breaks in DNA are primarily repaired by Non-Homologous End-Joining (NHEJ). The ring-shaped Ku heterodimer rapidly senses and threads onto broken DNA ends forming a recruiting hub. Through protein-protein contacts eventually reinforced by protein-DNA interactions, the Ku-DNA hub attracts a series of specialized proteins with scaffolding and/or enzymatic properties. To shed light on these dynamic interplays, we review here current knowledge on proteins directly interacting with Ku and on the contact points involved, with a particular accent on the different classes of Ku-binding motifs identified in several Ku partners. An integrated structural model of the core NHEJ network at the synapsis step is proposed.

Identifiants

pubmed: 30851288
pii: S0079-6107(18)30251-7
doi: 10.1016/j.pbiomolbio.2019.03.001
pii:
doi:

Substances chimiques

DNA 9007-49-2
Ku Autoantigen EC 4.2.99.-

Types de publication

Journal Article Research Support, Non-U.S. Gov't Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

62-76

Informations de copyright

Copyright © 2019 Elsevier Ltd. All rights reserved.

Auteurs

Philippe Frit (P)

Institut de Pharmacologie et Biologie Structurale, IPBS, Université de Toulouse, CNRS, UPS, Toulouse, France; Equipe Labellisée Ligue Contre le Cancer, Toulouse, France.

Virginie Ropars (V)

Institute for Integrative Biology of the Cell (I2BC), Institute Joliot, CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, 91198, Gif-sur-Yvette Cedex, France.

Mauro Modesti (M)

Cancer Research Center of Marseille, CNRS UMR7258, Inserm U1068, Institut Paoli-Calmettes, Aix-Marseille Université UM105, Marseille, France; Equipe Labellisée Ligue Contre le Cancer, Marseille, France.

Jean Baptiste Charbonnier (JB)

Institute for Integrative Biology of the Cell (I2BC), Institute Joliot, CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, 91198, Gif-sur-Yvette Cedex, France. Electronic address: jb.charbonnier@cea.fr.

Patrick Calsou (P)

Institut de Pharmacologie et Biologie Structurale, IPBS, Université de Toulouse, CNRS, UPS, Toulouse, France; Equipe Labellisée Ligue Contre le Cancer, Toulouse, France. Electronic address: calsou@ipbs.fr.

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Classifications MeSH