Image-Guided Transarterial Directed Delivery of Human Mesenchymal Stem Cells for Targeted Gastrointestinal Therapies in a Swine Model.
Animals
Catheterization, Peripheral
/ adverse effects
Duodenum
/ blood supply
Embolization, Therapeutic
Feasibility Studies
Female
Humans
Ischemia
/ etiology
Jejunum
/ blood supply
Mesenchymal Stem Cell Transplantation
/ adverse effects
Mesenteric Arteries
/ diagnostic imaging
Models, Animal
Radiography, Interventional
Risk Factors
Swine
Swine, Miniature
Journal
Journal of vascular and interventional radiology : JVIR
ISSN: 1535-7732
Titre abrégé: J Vasc Interv Radiol
Pays: United States
ID NLM: 9203369
Informations de publication
Date de publication:
Jul 2019
Jul 2019
Historique:
received:
15
08
2018
revised:
18
09
2018
accepted:
28
09
2018
pubmed:
11
3
2019
medline:
7
1
2020
entrez:
11
3
2019
Statut:
ppublish
Résumé
To evaluate the feasibility of catheter-directed intra-arterial stem cell delivery of human mesenchymal stem cells (MSCs) to the small bowel in a porcine model. The cranial mesenteric artery of 6 Yucatan minipigs was selectively catheterized under fluoroscopic guidance following cut-down and carotid artery access. A proximal jejunal branch artery was selectively catheterized for directed delivery of embolic microspheres (100-300 μm) or MSCs (0.1-10 million cells). The pigs were euthanized after 4 hours and specimens collected from the proximal duodenum and the targeted segment of the jejunum. The Chiu/Park system for scoring intestinal ischemia was used to compare hematoxylin and eosin-stained sections of jejunum and duodenum. Successful delivery of microspheres or MSCs in a proximal jejunal branch artery of the cranial mesenteric artery was achieved in all subjects. Radiopaque microspheres and post-delivery angiographic evidence of stasis in the targeted vessels were observed on fluoroscopy after delivery of embolics. Preserved blood flow was observed after MSC delivery in the targeted vessel. The Chiu/Park score for intestinal ischemia in the targeted proximal jejunal segments were similar for microspheres (4, 4; n = 2) and MSCs (4, 4, 4, 3; n = 4), indicating moderate ischemic effects that were greater than for control duodenal tissue (3, 1; 0, 0, 3, 3). Selective arteriographic deployment of MSCs in swine is feasible for study of directed intestinal stem cell delivery. In this study, directed therapy resulted in intestinal ischemia.
Identifiants
pubmed: 30852052
pii: S1051-0443(18)31581-1
doi: 10.1016/j.jvir.2018.09.034
pmc: PMC6589393
mid: NIHMS1523391
pii:
doi:
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1128-1134.e5Subventions
Organisme : NIAMS NIH HHS
ID : F30 AR069472
Pays : United States
Organisme : NIH HHS
ID : P51 OD011132
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM008169
Pays : United States
Informations de copyright
Copyright © 2018 SIR. Published by Elsevier Inc. All rights reserved.
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