LINE1 Derepression in Aged Wild-Type and SIRT6-Deficient Mice Drives Inflammation.


Journal

Cell metabolism
ISSN: 1932-7420
Titre abrégé: Cell Metab
Pays: United States
ID NLM: 101233170

Informations de publication

Date de publication:
02 04 2019
Historique:
received: 31 07 2018
revised: 02 11 2018
accepted: 22 02 2019
pubmed: 12 3 2019
medline: 2 6 2020
entrez: 12 3 2019
Statut: ppublish

Résumé

Mice deficient for SIRT6 exhibit a severely shortened lifespan, growth retardation, and highly elevated LINE1 (L1) activity. Here we report that SIRT6-deficient cells and tissues accumulate abundant cytoplasmic L1 cDNA, which triggers strong type I interferon response via activation of cGAS. Remarkably, nucleoside reverse-transcriptase inhibitors (NRTIs), which inhibit L1 retrotransposition, significantly improved health and lifespan of SIRT6 knockout mice and completely rescued type I interferon response. In tissue culture, inhibition of L1 with siRNA or NRTIs abrogated type I interferon response, in addition to a significant reduction of DNA damage markers. These results indicate that L1 activation contributes to the pathologies of SIRT6 knockout mice. Similarly, L1 transcription, cytoplasmic cDNA copy number, and type I interferons were elevated in the wild-type aged mice. As sterile inflammation is a hallmark of aging, we propose that modulating L1 activity may be an important strategy for attenuating age-related pathologies.

Identifiants

pubmed: 30853213
pii: S1550-4131(19)30120-2
doi: 10.1016/j.cmet.2019.02.014
pmc: PMC6449196
mid: NIHMS1522926
pii:
doi:

Substances chimiques

Dideoxynucleotides 0
ECAT11 protein, mouse 0
RNA-Binding Proteins 0
Thymine Nucleotides 0
Zidovudine 4B9XT59T7S
zidovudine triphosphate 6RGF96R053
Stavudine BO9LE4QFZF
Sirt6 protein, mouse EC 2.4.2.31
Sirtuins EC 3.5.1.-

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

871-885.e5

Subventions

Organisme : NIA NIH HHS
ID : R37 AG046320
Pays : United States
Organisme : NIA NIH HHS
ID : P01 AG047200
Pays : United States
Organisme : NIAMS NIH HHS
ID : R61 AR076807
Pays : United States
Organisme : NIA NIH HHS
ID : R01 AG027237
Pays : United States
Organisme : NIA NIH HHS
ID : R37 AG016694
Pays : United States
Organisme : NIA NIH HHS
ID : DP1 AG047745
Pays : United States
Organisme : NIA NIH HHS
ID : P01 AG051449
Pays : United States
Organisme : NIA NIH HHS
ID : R01 AG046320
Pays : United States

Informations de copyright

Copyright © 2019 Elsevier Inc. All rights reserved.

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Auteurs

Matthew Simon (M)

Department of Biology, University of Rochester, Rochester, NY 14627, USA.

Michael Van Meter (M)

Department of Biology, University of Rochester, Rochester, NY 14627, USA.

Julia Ablaeva (J)

Department of Biology, University of Rochester, Rochester, NY 14627, USA.

Zhonghe Ke (Z)

Department of Biology, University of Rochester, Rochester, NY 14627, USA.

Raul S Gonzalez (RS)

Department of Pathology, University of Rochester Medical Center, Rochester, NY 14627, USA.

Taketo Taguchi (T)

Department of Biology, University of Rochester, Rochester, NY 14627, USA.

Marco De Cecco (M)

Institute for Translational Research, Ariel University, Ariel, Israel.

Katerina I Leonova (KI)

Department of Cell Stress Biology, Roswell Park Comprehensive Cancer Center, Buffalo, NY 14263, USA.

Valeria Kogan (V)

Institute for Translational Research, Ariel University, Ariel, Israel.

Stephen L Helfand (SL)

Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI 02912, USA.

Nicola Neretti (N)

Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI 02912, USA.

Asael Roichman (A)

Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.

Haim Y Cohen (HY)

Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.

Margarita V Meer (MV)

Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Vadim N Gladyshev (VN)

Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Marina P Antoch (MP)

Department of Pharmacology and Therapeutics, Roswell Park Comprehensive Cancer Center, Buffalo, NY 14263, USA.

Andrei V Gudkov (AV)

Department of Cell Stress Biology, Roswell Park Comprehensive Cancer Center, Buffalo, NY 14263, USA.

John M Sedivy (JM)

Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI 02912, USA.

Andrei Seluanov (A)

Department of Biology, University of Rochester, Rochester, NY 14627, USA. Electronic address: andrei.seluanov@rochester.edu.

Vera Gorbunova (V)

Department of Biology, University of Rochester, Rochester, NY 14627, USA. Electronic address: vera.gorbunova@rochester.edu.

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Classifications MeSH