Macroencapsulated Human iPSC-Derived Pancreatic Progenitors Protect against STZ-Induced Hyperglycemia in Mice.
Animals
Biomarkers
Blood Glucose
C-Peptide
/ blood
Cell Differentiation
Diabetes Mellitus, Experimental
/ blood
Disease Models, Animal
Endoderm
/ cytology
Fluorescent Antibody Technique
Humans
Hyperglycemia
/ etiology
Immunophenotyping
Induced Pluripotent Stem Cells
/ cytology
Insulin
/ biosynthesis
Insulin-Secreting Cells
/ cytology
Mice
Models, Biological
Stem Cell Transplantation
Treatment Outcome
diabetes mellitus
differentiation
encapsulation
human
iPSC
stem cell
therapy
β cell
Journal
Stem cell reports
ISSN: 2213-6711
Titre abrégé: Stem Cell Reports
Pays: United States
ID NLM: 101611300
Informations de publication
Date de publication:
09 04 2019
09 04 2019
Historique:
received:
17
03
2018
revised:
05
02
2019
accepted:
06
02
2019
pubmed:
12
3
2019
medline:
21
4
2020
entrez:
12
3
2019
Statut:
ppublish
Résumé
In type 1 diabetes, a renewable source of human pancreatic β cells, in particular from human induced pluripotent stem cell (hiPSC) origin, would greatly benefit cell therapy. Earlier work showed that pancreatic progenitors differentiated from human embryonic stem cells in vitro can further mature to become glucose responsive following macroencapsulation and transplantation in mice. Here we took a similar approach optimizing the generation of pancreatic progenitors from hiPSCs. This work demonstrates that hiPSCs differentiated to pancreatic endoderm in vitro can be efficiently and robustly generated under large-scale conditions. The hiPSC-derived pancreatic endoderm cells (HiPECs) can further differentiate into glucose-responsive islet-like cells following macroencapsulation and in vivo implantation. The HiPECs can protect mice from streptozotocin-induced hyperglycemia and maintain normal glucose homeostasis and equilibrated plasma glucose concentrations at levels similar to the human set point. These results further validate the potential use of hiPSC-derived islet cells for application in clinical settings.
Identifiants
pubmed: 30853374
pii: S2213-6711(19)30050-5
doi: 10.1016/j.stemcr.2019.02.002
pmc: PMC6449839
pii:
doi:
Substances chimiques
Biomarkers
0
Blood Glucose
0
C-Peptide
0
Insulin
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
787-800Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.
Références
Nat Biotechnol. 2006 Nov;24(11):1392-401
pubmed: 17053790
PLoS One. 2012;7(5):e37004
pubmed: 22623968
Nat Biotechnol. 2014 Nov;32(11):1121-33
pubmed: 25211370
Nat Biotechnol. 2008 Apr;26(4):443-52
pubmed: 18288110
Proteomics Clin Appl. 2018 Sep;12(5):e1600173
pubmed: 29578310
Development. 2011 Mar;138(5):861-71
pubmed: 21270052
J Biol Chem. 2014 Mar 28;289(13):9182-94
pubmed: 24554722
N Engl J Med. 2000 Jul 27;343(4):230-8
pubmed: 10911004
Am J Physiol Endocrinol Metab. 2010 Nov;299(5):E713-20
pubmed: 20587750
Stem Cell Res. 2014 May;12(3):807-14
pubmed: 24788136
Nat Biotechnol. 2011 Jul 31;29(8):750-6
pubmed: 21804561
Diabetologia. 2001 Nov;44(11):2066-76
pubmed: 11719839
Transplantation. 2009 Apr 15;87(7):983-91
pubmed: 19352116
Cell Res. 2007 Apr;17(4):333-44
pubmed: 17426693
Transplantation. 1991 Jul;52(1):172-5
pubmed: 1830427
Nat Med. 2016 Mar;22(3):306-11
pubmed: 26808346
Diabetes. 2012 Aug;61(8):2016-29
pubmed: 22740171
Biomaterials. 2014 Jan;35(2):779-91
pubmed: 24103654
Cell. 2014 Oct 9;159(2):428-39
pubmed: 25303535
Nat Biotechnol. 2005 Dec;23(12):1534-41
pubmed: 16258519
Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):2930-3
pubmed: 14973194
Cell Res. 2009 Apr;19(4):429-38
pubmed: 19255591
Endocrinology. 2002 Jul;143(7):2491-5
pubmed: 12072379
Trends Endocrinol Metab. 2016 Mar;27(3):153-162
pubmed: 26774512
Biomaterials. 2011 Oct;32(28):6692-709
pubmed: 21715002
Am J Physiol Endocrinol Metab. 2014 Nov 1;307(9):E838-46
pubmed: 25205822
JAMA. 2005 Feb 16;293(7):830-5
pubmed: 15713772
Stem Cell Reports. 2015 Apr 14;4(4):591-604
pubmed: 25843049
Nat Commun. 2016 May 10;7:11463
pubmed: 27163171
EMBO J. 2015 Jul 2;34(13):1759-72
pubmed: 25908839
Transplant Proc. 2004 May;36(4):1108-10
pubmed: 15194386
Mol Cell Biol. 2005 Jun;25(12):4969-76
pubmed: 15923615
Stem Cell Reports. 2015 Dec 8;5(6):1081-1096
pubmed: 26677767
Stem Cell Reports. 2018 Mar 13;10(3):739-750
pubmed: 29503087
Stem Cells Transl Med. 2015 Aug;4(8):927-31
pubmed: 26062982
J Cell Sci. 1993 Jul;105 ( Pt 3):753-64
pubmed: 8408302
Curr Diab Rep. 2011 Oct;11(5):345-54
pubmed: 21830042
Cell Transplant. 2013;22(7):1137-46
pubmed: 23043940