Cytoskeletal actin patterns shape mast cell activation.
Actin Cytoskeleton
/ metabolism
Actins
/ metabolism
Animals
Biomarkers
Cell Degranulation
/ immunology
Cytoskeleton
/ metabolism
Fluorescent Antibody Technique
Leukemia, Basophilic, Acute
Mast Cells
/ immunology
Myosin Type II
/ metabolism
Protein Binding
Protein Transport
Rats
Receptors, IgE
/ metabolism
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
2019
2019
Historique:
received:
31
10
2018
accepted:
22
01
2019
entrez:
12
3
2019
pubmed:
12
3
2019
medline:
12
3
2019
Statut:
epublish
Résumé
Activation of immune cells relies on a dynamic actin cytoskeleton. Despite detailed knowledge of molecular actin assembly, the exact processes governing actin organization during activation remain elusive. Using advanced microscopy, we here show that Rat Basophilic Leukemia (RBL) cells, a model mast cell line, employ an orchestrated series of reorganization events within the cortical actin network during activation. In response to IgE antigen-stimulation of FCε receptors (FCεR) at the RBL cell surface, we observed symmetry breaking of the F-actin network and subsequent rapid disassembly of the actin cortex. This was followed by a reassembly process that may be driven by the coordinated transformation of distinct nanoscale F-actin architectures, reminiscent of self-organizing actin patterns. Actin patterns co-localized with zones of Arp2/3 nucleation, while network reassembly was accompanied by myosin-II activity. Strikingly, cortical actin disassembly coincided with zones of granule secretion, suggesting that cytoskeletal actin patterns contribute to orchestrate RBL cell activation.
Identifiants
pubmed: 30854485
doi: 10.1038/s42003-019-0322-9
pii: 10.1038/s42003-019-0322-9
pmc: PMC6405992
doi:
Substances chimiques
Actins
0
Biomarkers
0
Receptors, IgE
0
Myosin Type II
EC 3.6.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
93Subventions
Organisme : Medical Research Council
ID : MC_UP_1201/15
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_PC_15060
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UU_00008/9
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 212343/Z/18/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 104924/14/Z/14
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UU_12010/9
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Howard Hughes Medical Institute
Pays : United States
Organisme : Medical Research Council
ID : MR/K01577X/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 100262/Z/12/Z
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_PC_16082
Pays : United Kingdom
Déclaration de conflit d'intérêts
The authors declare no competing interests.
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