Claudin-1 inhibits human parainfluenza virus type 2 dissemination.


Journal

Virology
ISSN: 1096-0341
Titre abrégé: Virology
Pays: United States
ID NLM: 0110674

Informations de publication

Date de publication:
05 2019
Historique:
received: 09 10 2018
revised: 04 01 2019
accepted: 22 01 2019
pubmed: 12 3 2019
medline: 23 10 2019
entrez: 12 3 2019
Statut: ppublish

Résumé

Tight junctions enable epithelial cells to form physical barriers that act as an innate immune defense against respiratory infection. However, the involvement of tight junction molecules in paramyxovirus infections, which include various respiratory pathogens, has not been examined in detail. Human parainfluenza virus type 2 (hPIV2) infects airway epithelial cells and causes respiratory illness. In the present study, we found that hPIV2 infection of cultured cells induces expression of claudin-1 (CLDN1), an essential component of tight junctions. This induction seemed to be intrinsically restricted by V, an accessory protein that modulates various host responses, to enable efficient virus propagation. By generating CLDN1 over-expressing and knockout cell lines, we showed that CLDN1 is involved in the restriction of hPIV2 spread via cell-to-cell contact. Taken together, we identified CLDN1 an inhibitory factor for hPIV2 dissemination, and that its V protein acts to counter this.

Identifiants

pubmed: 30856486
pii: S0042-6822(19)30025-X
doi: 10.1016/j.virol.2019.01.031
pii:
doi:

Substances chimiques

Claudin-1 0
Viral Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

93-99

Informations de copyright

Copyright © 2019 Elsevier Inc. All rights reserved.

Auteurs

Natsuko Yumine (N)

Department of Microbiology, School of Medicine, Wakayama Medical University, Wakayama, Japan.

Yusuke Matsumoto (Y)

Department of Microbiology, School of Medicine, Wakayama Medical University, Wakayama, Japan.

Keisuke Ohta (K)

Department of Microbiology, School of Medicine, Wakayama Medical University, Wakayama, Japan.

Masayoshi Fukasawa (M)

Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Tokyo, Japan.

Machiko Nishio (M)

Department of Microbiology, School of Medicine, Wakayama Medical University, Wakayama, Japan. Electronic address: mnishio@wakayama-med.ac.jp.

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Classifications MeSH